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Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression.
DC Field | Value | Language |
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dc.contributor.author | 전경희 | - |
dc.date.accessioned | 2014-12-19T16:49:28Z | - |
dc.date.available | 2014-12-19T16:49:28Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90263 | - |
dc.description.abstract | Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic melanoma cell line, in which galectin-3 and matrix metalloproteinase- 1 (MMP-1) were both found to be highly expressed. Knockdown of galectin-3 with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of galectin-3 knockdown on the activity of AP-1, a transcriptional factor regulating MMP-1 expression. We found that galectin-3 directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1 transcription. Moreover, silencing of galectin-3 inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings, our in vivo study demonstrated that galectin-3 shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin- 3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1 and thereby up-regulating MMP-1 expression. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Binding Sites/genetics | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Movement | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Galectin 3/genetics | - |
dc.subject.MESH | Galectin 3/metabolism* | - |
dc.subject.MESH | JNK Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | Lung Neoplasms/drug therapy | - |
dc.subject.MESH | Lung Neoplasms/genetics | - |
dc.subject.MESH | Lung Neoplasms/secondary* | - |
dc.subject.MESH | Matrix Metalloproteinase 1/genetics* | - |
dc.subject.MESH | Matrix Metalloproteinase 1/metabolism* | - |
dc.subject.MESH | Melanoma, Experimental/metabolism* | - |
dc.subject.MESH | Melanoma, Experimental/pathology* | - |
dc.subject.MESH | Melanoma, Experimental/secondary | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | NIH 3T3 Cells | - |
dc.subject.MESH | Neoplasm Metastasis | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Proto-Oncogene Proteins c-fos/metabolism | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.subject.MESH | Transcription Factor AP-1/genetics* | - |
dc.subject.MESH | Transcription Factor AP-1/metabolism | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.subject.MESH | Transcriptional Activation | - |
dc.title | Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Yuan-Guo Wang | - |
dc.contributor.googleauthor | Seok-Jun Kim | - |
dc.contributor.googleauthor | Jung-Hwan Baek | - |
dc.contributor.googleauthor | Hyun-Woo Lee | - |
dc.contributor.googleauthor | Seo-Young Jeong | - |
dc.contributor.googleauthor | Kyung-Hee Chun | - |
dc.identifier.doi | 22437631 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03501 | - |
dc.relation.journalcode | J00860 | - |
dc.identifier.eissn | 2092-6413 | - |
dc.identifier.pmid | 22437631 | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Binding Sites/genetics | - |
dc.subject.keyword | Cell Line, Tumor | - |
dc.subject.keyword | Cell Movement | - |
dc.subject.keyword | Cell Proliferation | - |
dc.subject.keyword | Galectin 3/genetics | - |
dc.subject.keyword | Galectin 3/metabolism* | - |
dc.subject.keyword | JNK Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.keyword | Lung Neoplasms/drug therapy | - |
dc.subject.keyword | Lung Neoplasms/genetics | - |
dc.subject.keyword | Lung Neoplasms/secondary* | - |
dc.subject.keyword | Matrix Metalloproteinase 1/genetics* | - |
dc.subject.keyword | Matrix Metalloproteinase 1/metabolism* | - |
dc.subject.keyword | Melanoma, Experimental/metabolism* | - |
dc.subject.keyword | Melanoma, Experimental/pathology* | - |
dc.subject.keyword | Melanoma, Experimental/secondary | - |
dc.subject.keyword | Mice | - |
dc.subject.keyword | Mice, Inbred C57BL | - |
dc.subject.keyword | NIH 3T3 Cells | - |
dc.subject.keyword | Neoplasm Metastasis | - |
dc.subject.keyword | Promoter Regions, Genetic | - |
dc.subject.keyword | Proto-Oncogene Proteins c-fos/metabolism | - |
dc.subject.keyword | RNA Interference | - |
dc.subject.keyword | RNA, Small Interfering | - |
dc.subject.keyword | Transcription Factor AP-1/genetics* | - |
dc.subject.keyword | Transcription Factor AP-1/metabolism | - |
dc.subject.keyword | Transcription, Genetic | - |
dc.subject.keyword | Transcriptional Activation | - |
dc.contributor.alternativeName | Chun, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Chun, Kyung Hee | - |
dc.citation.volume | 44 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 387 | - |
dc.citation.endPage | 393 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.44(6) : 387-393, 2012 | - |
dc.identifier.rimsid | 34103 | - |
dc.type.rims | ART | - |
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