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Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

DC Field Value Language
dc.contributor.author박국인-
dc.date.accessioned2014-12-19T16:33:05Z-
dc.date.available2014-12-19T16:33:05Z-
dc.date.issued2012-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89743-
dc.description.abstractSurface modification of tissue engineering scaffolds and substrates is required for improving the efficacy of stem cell therapy by generating physicochemical stimulation promoting proliferation and differentiation of stem cells. However, typical surface modification methods including chemical conjugation or physical absorption have several limitations such as multistep, complicated procedures, surface denaturation, batch-to-batch inconsistencies, and low surface conjugation efficiency. In this study, we report a mussel-inspired, biomimetic approach to surface modification for efficient and reliable manipulation of human neural stem cell (NSC) differentiation and proliferation. Our study demonstrates that polydopamine coating facilitates highly efficient, simple immobilization of neurotrophic growth factors and adhesion peptides onto polymer substrates. The growth factor or peptide-immobilized substrates greatly enhance differentiation and proliferation of human NSCs (human fetal brain-derived NSCs and human induced pluripotent stem cell-derived NSCs) at a level comparable or greater than currently available animal-derived coating materials (Matrigel) with safety issues. Therefore, polydopamine-mediated surface modification can provide a versatile platform technology for developing chemically defined, safe, functional substrates and scaffolds for therapeutic applications of human NSCs.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBiomimetics/methods-
dc.subject.MESHCell Adhesion-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCoated Materials, Biocompatible/chemistry*-
dc.subject.MESHCollagen/chemistry-
dc.subject.MESHDrug Combinations-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry/methods-
dc.subject.MESHIndoles/chemistry*-
dc.subject.MESHLaminin/chemistry-
dc.subject.MESHMice-
dc.subject.MESHModels, Chemical-
dc.subject.MESHNeural Stem Cells/cytology*-
dc.subject.MESHNeurons/cytology-
dc.subject.MESHPeptides/chemistry-
dc.subject.MESHPolymers/chemistry*-
dc.subject.MESHProteoglycans/chemistry-
dc.subject.MESHSurface Properties-
dc.subject.MESHTissue Engineering/methods*-
dc.subject.MESHTissue Scaffolds/chemistry*-
dc.titlePolydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pediatrics (소아과학)-
dc.contributor.googleauthorKisuk Yang-
dc.contributor.googleauthorJung Seung Lee-
dc.contributor.googleauthorJin Kim-
dc.contributor.googleauthorYu Bin Lee-
dc.contributor.googleauthorHeungsoo Shin-
dc.contributor.googleauthorSoong Ho Um-
dc.contributor.googleauthorJeong Beom Kim-
dc.contributor.googleauthorKook In Park-
dc.contributor.googleauthorHaeshin Lee-
dc.contributor.googleauthorSeung-Woo Cho-
dc.identifier.doi10.1016/j.biomaterials.2012.06.067-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01438-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid22809643-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S014296121200717X-
dc.subject.keywordPolydopamine-
dc.subject.keywordSurface immobilization-
dc.subject.keywordNeural stem cell-
dc.subject.keywordNeurotrophic growth factor-
dc.subject.keywordAdhesion peptide-
dc.contributor.alternativeNamePark, Kook In-
dc.contributor.affiliatedAuthorPark, Kook In-
dc.citation.volume33-
dc.citation.number29-
dc.citation.startPage6952-
dc.citation.endPage6964-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.33(29) : 6952-6964, 2012-
dc.identifier.rimsid31879-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers

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