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Targeting ILK and β4 integrin abrogates the invasive potential of ovarian cancer

DC FieldValueLanguage
dc.contributor.author강숙희-
dc.contributor.author조남훈-
dc.contributor.author김백길-
dc.date.accessioned2014-12-19T16:32:08Z-
dc.date.available2014-12-19T16:32:08Z-
dc.date.issued2012-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89713-
dc.description.abstractIntegrins and integrin-linked kinase (ILK) are essential to cancerous invasion because they mediate physical interactions with the extracellular matrix, and regulate oncogenic signaling pathways. The purpose of our study is to determine whether deletion of β1 and β4 integrin and ILK, alone or in combination, has antitumoral effects in ovarian cancer. Expression of β1 and β4 integrin and ILK was analyzed by immunohistochemistry in 196 ovarian cancer tissue samples. We assessed the effects of depleting these molecules with shRNAs in ovarian cancer cells by Western blot, conventional RT-PCR, cell proliferation, migration, invasion, and in vitro Rac1 activity assays, and in vivo xenograft formation assays. Overexpression of β4 integrin and ILK in human ovarian cancer specimens was found to correlate with tumor aggressiveness. Depletion of these targets efficiently suppresses ovarian cancer cell proliferation, migration, and invasion in vitro and xenograft tumor formation in vivo. We also demonstrated that single depletion of ILK or combination depletion of β4 integrin/ILK inhibits phosphorylation of downstream signaling targets, p-Ser 473 Akt and p-Thr202/Tyr204 Erk1/2, and activation of Rac1, as well as reduce expression of MMP-2 and MMP-9 and increase expression of caspase-3 in vitro. In conclusion, targeting β4 integrin combined with ILK can instigate the latent tumorigenic potential and abrogate the invasive potential in ovarian cancer-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Movement/genetics-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCell Transformation, Neoplastic/genetics-
dc.subject.MESHCell Transformation, Neoplastic/pathology-
dc.subject.MESHFemale-
dc.subject.MESHGene Targeting*-
dc.subject.MESHGenes, Tumor Suppressor*-
dc.subject.MESHHumans-
dc.subject.MESHIntegrin beta4/genetics*-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHNeoplasm Invasiveness-
dc.subject.MESHNeoplasm Transplantation-
dc.subject.MESHOvarian Neoplasms/genetics-
dc.subject.MESHOvarian Neoplasms/pathology-
dc.subject.MESHOvarian Neoplasms/therapy*-
dc.subject.MESHProtein-Serine-Threonine Kinases/genetics*-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.titleTargeting ILK and β4 integrin abrogates the invasive potential of ovarian cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorYoon Pyo Choi-
dc.contributor.googleauthorBaek Gil Kim-
dc.contributor.googleauthorMing-Qing Gao-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorNam Hoon Cho-
dc.identifier.doi23026047-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00044-
dc.contributor.localIdA03812-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid23026047-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X12018736-
dc.subject.keywordOvarian cancer-
dc.subject.keywordILK (integrin-linked kinase)-
dc.subject.keywordβ4 Integrin-
dc.subject.keywordCombined effect-
dc.subject.keywordInvasiveness-
dc.contributor.alternativeNameKang, Suki-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.citation.volume427-
dc.citation.number3-
dc.citation.startPage642-
dc.citation.endPage648-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.427(3) : 642-648, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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