Cited 68 times in
Protocatechuic aldehyde inhibits migration and proliferation of vascular smooth muscle cells and intravascular thrombosis
DC Field | Value | Language |
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dc.contributor.author | 구철룡 | - |
dc.contributor.author | 이은직 | - |
dc.contributor.author | 조윤희 | - |
dc.date.accessioned | 2014-12-19T16:31:41Z | - |
dc.date.available | 2014-12-19T16:31:41Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89699 | - |
dc.description.abstract | The migration and proliferation of vascular smooth muscle cells (VSMCs) and formation of intravascular thrombosis play crucial roles in the development of atherosclerotic lesions. This study examined the effects of protocatechuic aldehyde (PCA), a compound isolated from the aqueous extract of the root of Salvia miltiorrhiza, an herb used in traditional Chinese medicine to treat a variety of vascular diseases, on the migration and proliferation of VSMCs and platelets due to platelet-derived growth factor (PDGF). DNA 5-bromo-2'-deoxy-uridine (BrdU) incorporation and wound-healing assays indicated that PCA significantly attenuated PDGF-induced proliferation and migration of VSMCs at a pharmacologically relevant concentration (100 μM). On a molecular level, we observed down-regulation of the phosphatidylinositol 3-kinase (PI3K)/Akt and the mitogen-activated protein kinase (MAPK) pathways, both of which regulate key enzymes associated with migration and proliferation. We also found that PCA induced S-phase arrest of the VSMC cell cycle and suppressed cyclin D2 expression. In addition, PCA inhibited PDGF-BB-stimulated reactive oxygen species production in VSMCs, indicating that PCA's antioxidant properties may contribute to its suppression of PDGF-induced migration and proliferation in VSMCs. Finally, PCA exhibited an anti-thrombotic effect related to its inhibition of platelet aggregation, confirmed with an aggregometer. Together, these findings suggest a potential therapeutic role of PCA in the treatment of atherosclerosis and angioplasty-induced vascular restenosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anticoagulants/pharmacology* | - |
dc.subject.MESH | Antioxidants/pharmacology* | - |
dc.subject.MESH | Benzaldehydes/pharmacology* | - |
dc.subject.MESH | Blood Platelets/drug effects | - |
dc.subject.MESH | Catechols/pharmacology* | - |
dc.subject.MESH | Cell Movement/drug effects* | - |
dc.subject.MESH | Cell Proliferation/drug effects* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | MAP Kinase Signaling System/drug effects | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Muscle, Smooth, Vascular/cytology | - |
dc.subject.MESH | Muscle, Smooth, Vascular/drug effects* | - |
dc.subject.MESH | Muscle, Smooth, Vascular/metabolism | - |
dc.subject.MESH | Myocytes, Smooth Muscle/drug effects* | - |
dc.subject.MESH | Myocytes, Smooth Muscle/metabolism | - |
dc.subject.MESH | Myocytes, Smooth Muscle/physiology | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Reactive Oxygen Species/antagonists & inhibitors | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | S Phase Cell Cycle Checkpoints/drug effects | - |
dc.subject.MESH | Thrombosis/blood | - |
dc.subject.MESH | Thrombosis/physiopathology* | - |
dc.title | Protocatechuic aldehyde inhibits migration and proliferation of vascular smooth muscle cells and intravascular thrombosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Chang Yoon Moon | - |
dc.contributor.googleauthor | Cheol Ryong Ku | - |
dc.contributor.googleauthor | Yoon Hee Cho | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.05.092 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00201 | - |
dc.contributor.localId | A03050 | - |
dc.contributor.localId | A03876 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 22640742 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X12009825 | - |
dc.subject.keyword | Protocatechuic aldehyde | - |
dc.subject.keyword | Anti-oxidant | - |
dc.subject.keyword | Atherosclerosis | - |
dc.subject.keyword | Migration | - |
dc.subject.keyword | Proliferation | - |
dc.subject.keyword | Anti-platelet | - |
dc.subject.keyword | PDGF | - |
dc.contributor.alternativeName | Ku, Cheol Ryong | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.alternativeName | Cho, Yoon Hee | - |
dc.contributor.affiliatedAuthor | Ku, Cheol Ryong | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Cho, Yoon Hee | - |
dc.citation.volume | 423 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 116 | - |
dc.citation.endPage | 121 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.423(1) : 116-121, 2012 | - |
dc.identifier.rimsid | 31859 | - |
dc.type.rims | ART | - |
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