Cited 29 times in
Mitogen-activated protein kinase phosphatase-1 modulates regional effects of injurious mechanical ventilation in rodent lungs
DC Field | Value | Language |
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dc.contributor.author | 박무석 | - |
dc.date.accessioned | 2014-12-19T16:23:41Z | - |
dc.date.available | 2014-12-19T16:23:41Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1073-449X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89443 | - |
dc.description.abstract | RATIONALE: Mechanical ventilation induces heterogeneous lung injury by mitogen-activated protein kinase (MAPK) and nuclear factor-κB. Mechanisms regulating regional injury and protective effects of prone positioning are unclear. OBJECTIVES: To determine the key regulators of the lung regional protective effects of prone positioning in rodent lungs exposed to injurious ventilation. METHODS: Adult rats were ventilated with high (18 ml/kg, positive end-expiratory pressure [PEEP] 0) or low Vt (6 ml/kg; PEEP 3 cm H(2)O; 3 h) in supine or prone position. Dorsal-caudal lung mRNA was analyzed by microarray and MAPK phosphatases (MKP)-1 quantitative polymerase chain reaction. MKP-1(-/-) or wild-type mice were ventilated with very high (24 ml/kg; PEEP 0) or low Vt (6-7 ml/kg; PEEP 3 cm H(2)O). The MKP-1 regulator PG490-88 (MRx-108; 0.75 mg/kg) or phosphate-buffered saline was administered preventilation. Injury was assessed by lung mechanics, bronchioalveolar lavage cell counts, protein content, and lung injury scoring. Immunoblotting for MKP-1, and IκBα and cytokine ELISAs were performed on lung lysates. MEASUREMENTS AND MAIN RESULTS: Prone positioning was protective against injurious ventilation in rats. Expression profiling demonstrated MKP-1 20-fold higher in rats ventilated prone rather than supine and regional reduction in p38 and c-jun N-terminal kinase activation. MKP-1(-/-) mice experienced amplified injury. PG490-88 improved static lung compliance and injury scores, reduced bronchioalveolar lavage cell counts and cytokine levels, and induced MKP-1 and IκBα. CONCLUSIONS: Injurious ventilation induces MAPK in an MKP-1-dependent fashion. Prone positioning is protective and induces MKP-1. PG490-88 induced MKP-1 and was protective against high Vt in a nuclear factor-κB-dependent manner. MKP-1 is a potential target for modulating regional effects of injurious ventilation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Diterpenes/therapeutic use | - |
dc.subject.MESH | Down-Regulation/physiology | - |
dc.subject.MESH | Dual Specificity Phosphatase 1/physiology* | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | I-kappa B Proteins/metabolism | - |
dc.subject.MESH | Lung/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Prone Position/physiology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Respiration, Artificial/adverse effects* | - |
dc.subject.MESH | Supine Position/physiology | - |
dc.subject.MESH | Up-Regulation/physiology | - |
dc.subject.MESH | Ventilator-Induced Lung Injury/metabolism* | - |
dc.subject.MESH | Ventilator-Induced Lung Injury/prevention & control* | - |
dc.title | Mitogen-activated protein kinase phosphatase-1 modulates regional effects of injurious mechanical ventilation in rodent lungs | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Moo Suk Park | - |
dc.contributor.googleauthor | Qianbin He | - |
dc.contributor.googleauthor | Michael G. Edwards | - |
dc.contributor.googleauthor | Amen Sergew | - |
dc.contributor.googleauthor | David W. H. Riches | - |
dc.contributor.googleauthor | Richard K. Albert | - |
dc.contributor.googleauthor | Ivor S. Douglas | - |
dc.identifier.doi | 10.1164/rccm.201109-1593OC | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01457 | - |
dc.relation.journalcode | J00112 | - |
dc.identifier.eissn | 1535-4970 | - |
dc.identifier.pmid | 22582160 | - |
dc.subject.keyword | regionally injurious ventilation | - |
dc.subject.keyword | prone position | - |
dc.subject.keyword | rodent lung injury | - |
dc.subject.keyword | MKP-1/DUSP-1 | - |
dc.subject.keyword | triptolide | - |
dc.contributor.alternativeName | Park, Moo Suk | - |
dc.contributor.affiliatedAuthor | Park, Moo Suk | - |
dc.citation.volume | 186 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 72 | - |
dc.citation.endPage | 81 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol.186(1) : 72-81, 2012 | - |
dc.identifier.rimsid | 31771 | - |
dc.type.rims | ART | - |
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