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Expression of 12 cytokines in aqueous humour of uveal melanoma before and after combined Ruthenium-106 brachytherapy and transpupillary thermotherapy

 Christopher Seungkyu Lee  ;  Ik Hyun Jun  ;  Tae-im Kim  ;  Suk Ho Byeon  ;  Hyoung Jun Koh  ;  Sung Chul Lee 
 ACTA OPHTHALMOLOGICA, Vol.90(4) : 314-320, 2012 
Journal Title
Issue Date
Adult ; Aged ; Aqueous Humor/metabolism* ; Brachytherapy* ; Combined Modality Therapy ; Cytokines/metabolism* ; Eye Proteins/metabolism* ; Female ; Humans ; Hyperthermia, Induced* ; Male ; Melanoma/metabolism* ; Melanoma/radiotherapy ; Melanoma/therapy ; Middle Aged ; Protein Array Analysis ; Ruthenium Radioisotopes/therapeutic use ; Uveal Neoplasms/metabolism* ; Uveal Neoplasms/radiotherapy ; Uveal Neoplasms/therapy
angiogenesis ; brachytherapy ; cytokine ; inflammation ; uveal melanoma
PURPOSE: To determine the aqueous humour levels of 12 cytokines in eyes with uveal melanoma and whether their expression changes after combined Ru-106 brachytherapy and transpupillary thermotherapy (TTT).

METHODS: Aqueous humour samples were collected from 20 patients with previously untreated uveal melanoma undergoing combined Ru-106 brachytherapy and TTT, both at the time of plaque placement and removal. Using multiplex biochip array technology, 12 different cytokines were measured, including interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, vascular endothelial growth factor (VEGF), tumour necrosis factor (TNF)-α, interferon (IFN)-γ, epidermal growth factor (EGF) and monocyte chemoattractant protein (MCP)-1. Aqueous humour from 20 patients undergoing cataract surgery was used as control.
RESULTS: IL-6, IL-8, IFN-γ and MCP-1 were highly expressed in uveal melanoma, whereas IL-2, IL-10 and TNF- α were low in expression. There was a positive correlation between tumour height and IL-8 level (p = 0.020). Vascular endothelial growth factor tends to be highly expressed in melanoma-containing eyes (p = 0.056). Levels of IL-6, IL-8 and IL-1β increased after the mean 117 ± 38 hrs of brachytherapy and adjunctive TTT with a tumour apex dose of 61 ± 28 Gy and a scleral contact dose of 786 ± 226 Gy. Increase in levels of IL-6 (p = 0.003) and IL-8 (p = 0.046) positively correlated with scleral contact dose.
CONCLUSIONS: Cytokines such as IL-6, IL-8, IFN-γ and MCP-1 may be implicated in the progression of uveal melanoma. Ocular irradiation from a Ru-106 plaque promoted an increase in the levels of IL-6, IL-8 and IL-1β, modulation of which could be useful in managing radiation-related side effects.
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1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Koh, Hyoung Jun(고형준) ORCID logo https://orcid.org/0000-0002-5932-8516
Kim, Tae-Im(김태임) ORCID logo https://orcid.org/0000-0001-6414-3842
Byeon, Suk Ho(변석호) ORCID logo https://orcid.org/0000-0001-8101-0830
Lee, Sung Chul(이성철) ORCID logo https://orcid.org/0000-0001-9438-2385
Lee, Christopher Seungkyu(이승규) ORCID logo https://orcid.org/0000-0001-5054-9470
Jun, Ik Hyun(전익현) ORCID logo https://orcid.org/0000-0002-2160-1679
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