Since cardiovascular homografts were introduced in 1965, various types of processing techniques have been developed. Currently, cryopreservation technique using cryoprotectant, controlled freezing rate and storage in liquid nitrogen container is most commonly used. Although cardiovascular homograft has disadvantage of degenerative changes such as fibrosis and calcification, but it has been used selectively as a substitute for cardiac valve or vascular surgery due to hemodynamic excellence and resistance to infection.