Cited 124 times in
A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김어수 | - |
dc.date.accessioned | 2014-12-18T09:51:48Z | - |
dc.date.available | 2014-12-18T09:51:48Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88970 | - |
dc.description.abstract | We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-β (Aβ) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. Aβ at the level of 1 pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma Aβ using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alzheimer Disease/blood | - |
dc.subject.MESH | Amyloid beta-Peptides/blood* | - |
dc.subject.MESH | Bacterial Outer Membrane Proteins/chemistry | - |
dc.subject.MESH | Biosensing Techniques/instrumentation* | - |
dc.subject.MESH | Equipment Design | - |
dc.subject.MESH | Escherichia coli/chemistry | - |
dc.subject.MESH | Escherichia coli Proteins/chemistry | - |
dc.subject.MESH | Gold/chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Limit of Detection | - |
dc.subject.MESH | Nanotubes, Carbon/chemistry* | - |
dc.subject.MESH | Semiconductors* | - |
dc.title | A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Psychiatry (정신과학) | - |
dc.contributor.googleauthor | Jeseung Oh | - |
dc.contributor.googleauthor | Gu Yoo | - |
dc.contributor.googleauthor | Young Wook Chang | - |
dc.contributor.googleauthor | Hyung Joon Kim | - |
dc.contributor.googleauthor | Joachim Jose | - |
dc.contributor.googleauthor | Eosu Kim | - |
dc.contributor.googleauthor | Jae-Chul Pyun | - |
dc.contributor.googleauthor | Kyung-Hwa Yoo | - |
dc.identifier.doi | 10.1016/j.bios.2013.07.004 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00686 | - |
dc.relation.journalcode | J00330 | - |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.pmid | 23891796 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0956566313004740 | - |
dc.subject.keyword | Alzheimer's disease | - |
dc.subject.keyword | Amyloid-β | - |
dc.subject.keyword | Biosensor | - |
dc.subject.keyword | Carbon nanotube | - |
dc.subject.keyword | E. coli outer membrane with autodisplayed Z-domain | - |
dc.subject.keyword | Human serum | - |
dc.subject.keyword | Metal semiconductor field effect transistor | - |
dc.contributor.alternativeName | Kim, Eo Su | - |
dc.contributor.affiliatedAuthor | Kim, Eo Su | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 50 | - |
dc.citation.startPage | 345 | - |
dc.citation.endPage | 350 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, Vol.50 : 345-350, 2013 | - |
dc.identifier.rimsid | 33711 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.