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A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum

 Jeseung Oh  ;  Gu Yoo  ;  Young Wook Chang  ;  Hyung Joon Kim  ;  Joachim Jose  ;  Eosu Kim  ;  Jae-Chul Pyun  ;  Kyung-Hwa Yoo 
 Biosensors & Bioelectronics, Vol.50 : 345-350, 2013 
Journal Title
 Biosensors & Bioelectronics 
Issue Date
Alzheimer Disease/blood ; Amyloid beta-Peptides/blood* ; Bacterial Outer Membrane Proteins/chemistry ; Biosensing Techniques/instrumentation* ; Equipment Design ; Escherichia coli/chemistry ; Escherichia coli Proteins/chemistry ; Gold/chemistry ; Humans ; Limit of Detection ; Nanotubes, Carbon/chemistry* ; Semiconductors*
Alzheimer's disease ; Amyloid-β ; Biosensor ; Carbon nanotube ; E. coli outer membrane with autodisplayed Z-domain ; Human serum ; Metal semiconductor field effect transistor
We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-β (Aβ) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. Aβ at the level of 1 pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma Aβ using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease.
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1. College of Medicine (의과대학) > Dept. of Psychiatry (정신과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eosu(김어수) ORCID logo https://orcid.org/0000-0001-9472-9465
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