4 308

Cited 5 times in

Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6 expression in splenocytes

Authors
 Dong-In Koh  ;  Jae-Hyeon Yoon  ;  Min-Kyeong Kim  ;  Haemin An  ;  Min-Young Kim  ;  Man-Wook Hur 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.442(3-4) : 177-182, 2013 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2013
MeSH
Animals ; Cell Proliferation ; Gene Expression Regulation* ; Germinal Center/pathology* ; Mice ; Mice, Knockout ; Nuclear Receptor Co-Repressor 1/metabolism ; Organ Size ; Proto-Oncogene Proteins c-bcl-6/genetics* ; Repressor Proteins/genetics ; Repressor Proteins/metabolism* ; Spleen/pathology* ; Splenomegaly/genetics ; Splenomegaly/pathology* ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Transcription, Genetic
Keywords
B cell ; B-cell lymphoma 6 protein ; BCL6 ; Bcl6 ; Cell proliferation ; DLBCL ; Diffuse large B-cell lymphoma ; GC ; Germinal center ; Kaiso ; Spleen ; Splenomegaly ; germinal center
Abstract
Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso knockout mice, Bcl6 (a transcriptional repressor that plays a critical role in GC development in spleen) and c-Myc were highly expressed, while the cell cycle arrest genes p27 (CDKN1B), p21 (CDKN1A) and Gadd45a were downregulated. Chromatin immunoprecipitation (ChIP) and transcription assays suggested that Kaiso represses Bcl6 expression, and in Kaiso knockout mice, derepressed Bcl6 increased cell proliferation by suppressing p27 (CDKN1B), p21 (CDKN1A) and Gadd45a, while upregulating the oncogene c-Myc. Further evidence for Kaiso regulation of splenomegaly was provided by B lymphocyte Ramos cells, in which ectopic KAISO repressed BCL6 and c-MYC expression, while concomitantly increasing the expression of the cell cycle arrestors p21, p27 and Gadd45a. In summary, derepressed Bcl6 expression may be responsible for increases in GC cell proliferation and splenomegaly of Kaiso knockout mice.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X13019281
DOI
10.1016/j.bbrc.2013.11.046
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88880
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse