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Fast temporal detection of intracellular hydrogen peroxide by HyPer

Authors
 Yu-Mi Yang  ;  Sung Jun Lee  ;  Dong Min Shin 
Citation
 International Journal of Oral Biology, Vol.38(4) : 169-173, 2013 
Journal Title
 International Journal of Oral Biology 
ISSN
 1226-7155 
Issue Date
2013
Keywords
HyPer ; H2O2 ; ROS ; high speed detection
Abstract
HyPer is the genetically encoded biosensor of intracellular hydrogen peroxide (H2O2), the most stable of the reactive oxygen species (ROS) generated by living cells. HyPer has a high sensitivity and specificity for detecting intracellular H2O2 by confocal laser microscopy. However, it was not known whether high speed ratiometric imaging of H2O2 by HyPer is possible. We thus investigated the sensitivity of HyPer in detecting changes to the intracellular H2O2 levels in HEK293 and PC12 cells using a microfluorometer imaging system. Increase in the HyPer ratio were clearly evident on stimulations of more than 100 μM H2O2 and fast changes in the HyPer ratio were observed on ratiometric fluorescent images after H2O2 treatment. These results suggest that HyPer is a potent biosensor of the fast temporal production of intracellular H2O2.
Files in This Item:
T201304308.pdf Download
DOI
10.11620/IJOB.2013.38.4.169
Appears in Collections:
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Yang, Yu Mi(양유미)
Lee, Sung Jun(이성준)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88610
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