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MicroRNA-23b Functions as a Tumor Suppressor by Regulating Zeb1 in Bladder Cancer

DC FieldValueLanguage
dc.contributor.author장인익-
dc.date.accessioned2014-12-18T09:39:18Z-
dc.date.available2014-12-18T09:39:18Z-
dc.date.issued2013-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88577-
dc.description.abstractMicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression by targeted repression of transcription and translation. In this study we show that miRNA-23b (miR-23b) acts as a tumor suppressor in bladder cancer. Quantitative real-time PCR analysis showed that miR-23b is significantly down-regulated in bladder cancer cell lines and tumor tissues compared to non-malignant cells and normal tissue samples. We also demonstrate that miR-23b expression has a potential to be diagnostic and prognostic biomarker in bladder cancer. High miR-23b expression is positively correlated with higher overall survival of bladder cancer patients as revealed by Kaplan-Meier analysis. ROC analysis showed that miR-23b expression can distinguish between normal and bladder cancer tissues. Further we elucidated the biological significance of miR-23b in bladder cancer. Over-expression of miR-23b in bladder cancer cells inhibited cell proliferation and impaired colony formation. Fluorescence activated cell sorting (FACS) analysis revealed that re-expression of miR-23b in bladder cancer cells induced G0/G1 cell cycle arrest and apoptosis while inhibiting cell migration and invasion. Luciferase reporter assays demonstrated that Zeb1, a crucial regulator of epithelial-to-mesenchymal transition (EMT), is a direct target of miR-23b in bladder cancer. These results show that loss of miR-23b confers a proliferative advantage and promotes bladder cancer cell migration and invasion. Furthermore, re-expression of miR-23b may be a beneficial therapeutic strategy for the treatment of human bladder cancer.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMicroRNA-23b Functions as a Tumor Suppressor by Regulating Zeb1 in Bladder Cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorShahana Majid-
dc.contributor.googleauthorAltaf A. Dar-
dc.contributor.googleauthorSharanjot Saini-
dc.contributor.googleauthorGuoren Deng-
dc.contributor.googleauthorInik Chang-
dc.contributor.googleauthorKirsten Greene-
dc.contributor.googleauthorYuichiro Tanaka-
dc.contributor.googleauthorRajvir Dahiya-
dc.contributor.googleauthorSoichiro Yamamura-
dc.identifier.doi10.1371/journal.pone.0067686-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03461-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.contributor.alternativeNameChang, In Ik-
dc.contributor.affiliatedAuthorChang, In Ik-
dc.rights.accessRightsfree-
dc.citation.volume8-
dc.citation.number7-
dc.citation.startPagee67686-
dc.identifier.bibliographicCitationPLOS ONE, Vol.8(7) : e67686, 2013-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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