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Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor

Authors
 Seung-Hyun Yang  ;  Dan Heo  ;  Eugene Lee  ;  Eunjung Kim  ;  Eun-Kyung Lim  ;  Young Han Lee  ;  Seungjoo Haam  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Jaemoon Yang  ;  Sahng Wook Park 
Citation
 Nanotechnology, Vol.24(47) : 475103, 2013 
Journal Title
 Nanotechnology 
ISSN
 0957-4484 
Issue Date
2013
MeSH
Asialoglycoprotein Receptor/metabolism* ; Colloids/chemistry ; Ferric Compounds/metabolism* ; Fluorescence ; Galactose/metabolism* ; Glycosylation ; Hep G2 Cells ; Humans ; MCF-7 Cells ; Magnetic Resonance Imaging* ; Manganese Compounds/metabolism* ; Nanoparticles/chemistry* ; Nanoparticles/ultrastructure ; Particle Size ; Spectrophotometry, Atomic ; Spectroscopy, Fourier Transform Infrared ; Static Electricity
Keywords
Asialoglycoprotein Receptor/metabolism* ; Colloids/chemistry ; Ferric Compounds/metabolism* ; Fluorescence ; Galactose/metabolism* ; Glycosylation ; Hep G2 Cells ; Humans ; MCF-7 Cells ; Magnetic Resonance Imaging* ; Manganese Compounds/metabolism* ; Nanoparticles/chemistry* ; Nanoparticles/ultrastructure ; Particle Size ; Spectrophotometry, Atomic ; Spectroscopy, Fourier Transform Infrared ; Static Electricity
Abstract
Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.
Full Text
http://iopscience.iop.org/0957-4484/24/47/475103/
DOI
10.1088/0957-4484/24/47/475103
Appears in Collections:
5. Research Institutes (연구소) > Institute for Medical Convergence (연의-생공연 메디컬융합연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Seung Hyun(양승현)
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Lee, Young Han(이영한) ORCID logo https://orcid.org/0000-0002-5602-391X
Lee, Eu Gene(이유진)
Lim, Eun Kyung(임은경)
Heo, Dan(허단)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88526
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