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Mitogenesis of Vascular Smooth Muscle Cell Stimulated by Platelet-Derived Growth Factor-bb Is Inhibited by Blocking of Intracellular Signaling by Epigallocatechin-3-O-Gallate

Authors
 Mi Hee Lee  ;  Byeong-Ju Kwon  ;  Min-Ah Koo  ;  Kyung Eun You  ;  Jong-Chul Park 
Citation
 OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Vol.2013 : 827905, 2013 
Journal Title
 OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 
ISSN
 1942-0900 
Issue Date
2013
Abstract
Epigallocatechin gallate (EGCG) is known to exhibit antioxidant, antiproliferative, and antithrombogenic effects and reduce the risk of cardiovascular diseases. Key events in the development of cardiovascular disease are hypertrophy and hyperplasia according to vascular smooth muscle cell proliferation. In this study, we investigated whether EGCG can interfere with PDGF-bb stimulated proliferation, cell cycle distribution, and the gelatinolytic activity of MMP and signal transduction pathways on RAOSMC when it was treated in two different ways—cotreatment with PDGF-bb and pretreatment of EGCG before addition of PDGF-bb. Both cotreated and pretreated EGCG significantly inhibited PDGF-bb induced proliferation, cell cycle progression of the G0/G1 phase, and the gelatinolytic activity of MMP-2/9 on RAOSMC. Also, EGCG blocked PDGF receptor- (PDGFR-) phosphorylation on PDGF-bb stimulated RAOSMC under pretreatment with cells as well as cotreatment with PDGF-bb. The downstream signal transduction pathways of PDGFR-, including p42/44 MAPK, p38 MAPK, and Akt phosphorylation, were also inhibited by EGCG in a pattern similar to PDGFR- phosphorylation. These findings suggest that EGCG can inhibit PDGF-bb stimulated mitogenesis by indirectly and directly interrupting PDGF-bb signals and blocking the signaling pathway via PDGFR- phosphorylation. Furthermore, EGCG may be used for treatment and prevention of cardiovascular disease through blocking of PDGF-bb signaling.
Files in This Item:
T201304060.pdf Download
DOI
10.1155/2013/827905
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Kwon, Byeong-Ju(권병주) ORCID logo https://orcid.org/0000-0001-9916-0546
You, Kyung Eun(류경은)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88487
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