Cited 32 times in
Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories
DC Field | Value | Language |
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dc.contributor.author | 이경원 | - |
dc.date.accessioned | 2014-12-18T09:07:35Z | - |
dc.date.available | 2014-12-18T09:07:35Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0022-2615 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87583 | - |
dc.description.abstract | We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2 % of the staphylococci (95.9 % of coagulase-negative staphylococci), 97.8 % of the streptococci, and 100 % of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF MEDICAL MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bacteriological Techniques/methods | - |
dc.subject.MESH | Enterococcus faecalis/genetics | - |
dc.subject.MESH | Enterococcus faecalis/isolation & purification* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Laboratories/standards | - |
dc.subject.MESH | RNA, Bacterial/genetics | - |
dc.subject.MESH | RNA, Ribosomal, 16S/genetics | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods* | - |
dc.subject.MESH | Staphylococcus aureus/genetics | - |
dc.subject.MESH | Staphylococcus aureus/isolation & purification* | - |
dc.subject.MESH | Streptococcus agalactiae/genetics | - |
dc.subject.MESH | Streptococcus agalactiae/isolation & purification* | - |
dc.title | Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Hee-Won Moon | - |
dc.contributor.googleauthor | Sun Hwa Lee | - |
dc.contributor.googleauthor | Hae-Sun Chung | - |
dc.contributor.googleauthor | Miae Lee | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.identifier.doi | 10.1099/jmm.0.062950-0 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02649 | - |
dc.relation.journalcode | J01583 | - |
dc.identifier.eissn | 1473-5644 | - |
dc.identifier.pmid | 23764744 | - |
dc.identifier.url | http://jmm.sgmjournals.org/content/62/Pt_9/1301.long | - |
dc.subject.keyword | Bacteriological Techniques/methods | - |
dc.subject.keyword | Enterococcus faecalis/genetics | - |
dc.subject.keyword | Enterococcus faecalis/isolation & purification* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Laboratories/standards | - |
dc.subject.keyword | RNA, Bacterial/genetics | - |
dc.subject.keyword | RNA, Ribosomal, 16S/genetics | - |
dc.subject.keyword | Reproducibility of Results | - |
dc.subject.keyword | Sensitivity and Specificity | - |
dc.subject.keyword | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods* | - |
dc.subject.keyword | Staphylococcus aureus/genetics | - |
dc.subject.keyword | Staphylococcus aureus/isolation & purification* | - |
dc.subject.keyword | Streptococcus agalactiae/genetics | - |
dc.subject.keyword | Streptococcus agalactiae/isolation & purification* | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 62 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1301 | - |
dc.citation.endPage | 1306 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICAL MICROBIOLOGY, Vol.62(9) : 1301-1306, 2013 | - |
dc.identifier.rimsid | 34329 | - |
dc.type.rims | ART | - |
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