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Modified Midi- and Mini-Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

DC Field Value Language
dc.contributor.author이환영-
dc.contributor.author김나영-
dc.contributor.author신경진-
dc.contributor.author양우익-
dc.date.accessioned2014-12-18T09:04:34Z-
dc.date.available2014-12-18T09:04:34Z-
dc.date.issued2013-
dc.identifier.issn0022-1198-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/87487-
dc.description.abstractTwo multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60-year-old and 400-500-year-old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF FORENSIC SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHDNA Degradation, Necrotic*-
dc.subject.MESHDNA Primers-
dc.subject.MESHDNA, Mitochondrial/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHMultiplex Polymerase Chain Reaction/methods*-
dc.subject.MESHSequence Analysis, DNA/methods*-
dc.titleModified Midi- and Mini-Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorNa Young Kim-
dc.contributor.googleauthorHwan Young Lee-
dc.contributor.googleauthorSun Joo Park-
dc.contributor.googleauthorWoo Ick Yang-
dc.contributor.googleauthorKyoung-Jin Shin-
dc.identifier.doi10.1111/1556-4029.12062-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03335-
dc.contributor.localIdA02085-
dc.contributor.localIdA02300-
dc.contributor.localIdA00349-
dc.relation.journalcodeJ01413-
dc.identifier.eissn1556-4029-
dc.identifier.pmid23406419-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/1556-4029.12062/abstract-
dc.subject.keywordDNA Degradation, Necrotic*-
dc.subject.keywordDNA Primers-
dc.subject.keywordDNA, Mitochondrial/genetics*-
dc.subject.keywordHumans-
dc.subject.keywordMultiplex Polymerase Chain Reaction/methods*-
dc.subject.keywordSequence Analysis, DNA/methods*-
dc.contributor.alternativeNameLee, Hwan Young-
dc.contributor.alternativeNameKim, Na Young-
dc.contributor.alternativeNameShin, Kyoung Jin-
dc.contributor.alternativeNameYang, Woo Ick-
dc.contributor.affiliatedAuthorLee, Hwan Young-
dc.contributor.affiliatedAuthorShin, Kyoung Jin-
dc.contributor.affiliatedAuthorYang, Woo Ick-
dc.contributor.affiliatedAuthorKim, Na Young-
dc.rights.accessRightsnot free-
dc.citation.volume58-
dc.citation.number3-
dc.citation.startPage738-
dc.citation.endPage743-
dc.identifier.bibliographicCitationJOURNAL OF FORENSIC SCIENCES, Vol.58(3) : 738-743, 2013-
dc.identifier.rimsid34257-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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