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Modified Midi- and Mini-Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

Authors
 Na Young Kim  ;  Hwan Young Lee  ;  Sun Joo Park  ;  Woo Ick Yang  ;  Kyoung-Jin Shin 
Citation
 JOURNAL OF FORENSIC SCIENCES, Vol.58(3) : 738-743, 2013 
Journal Title
JOURNAL OF FORENSIC SCIENCES
ISSN
 0022-1198 
Issue Date
2013
MeSH
DNA Degradation, Necrotic* ; DNA Primers ; DNA, Mitochondrial/genetics* ; Humans ; Multiplex Polymerase Chain Reaction/methods* ; Sequence Analysis, DNA/methods*
Keywords
DNA Degradation, Necrotic* ; DNA Primers ; DNA, Mitochondrial/genetics* ; Humans ; Multiplex Polymerase Chain Reaction/methods* ; Sequence Analysis, DNA/methods*
Abstract
Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60-year-old and 400-500-year-old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/1556-4029.12062/abstract
DOI
10.1111/1556-4029.12062
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Na Young(김나영)
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87487
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