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Effects of mineral trioxide aggregate on the proliferation and differentiation of human dental pulp stromal cells from permanent and deciduous teeth

Other Titles
 Mineral trioxide aggregate가 유치 및 영구치의 치수기질세포 증식 및 분화에 미치는 영향 
 Kim, Seunghye  ;  Jeon, Mijeong  ;  Shin, Dong Min  ;  Lee, Jae Ho  ;  Song, Je Seon 
 Journal of the Korean Academy of Pediatric Dentistry (대한소아치과학회지), Vol.40(3) : 158-166, 2013 
Journal Title
Journal of the Korean Academy of Pediatric Dentistry(대한소아치과학회지)
Issue Date
Mineral Trioxide Aggregate (MTA) ; Human dental pulp stromal cells ; Deciduous teeth ; Permanent teeth ; Proliferation ; Differentiation
Mineral trioxide aggregate (MTA) has recently been used as a pulpotomy medicament for primary molars. The aim of this study was to evaluate and compare the proliferation and differentiation potential of dental pulp stromal cells of permanent teeth and deciduous teeth cultured on MTA-coated surface. Human dental pulp stromal cells were obtained from human permanent premolars and deciduous teeth and cultured on MTA-coated culture plates. The cells were subjected to proliferation assay and cell cycle analysis. Their differentiation potential was evaluated by analysing changes in the mRNA expressions of runt-related transcriptional factor 2 (Runx2) and alkaline phosphatase (ALP). Morphological changes of cells in direct contact with MTA were observed using scanning electron microscopy (SEM). The proliferation rates, distribution of cell cycles and mRNA expression patterns of Runx2 and ALP were similar in both types of pulpal cells. SEM observations revealed that both types changed into more dendrite-like cells. On the surface of MTA, human dental pulp stromal cells from deciduous and permanent teeth were able to both proliferate and differentiate into cells that induce mineralization. MTA is suitable as a biocompatible pulpotomy medicament for primary teeth.
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2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Hye(김승혜)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
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