Cited 4 times in
The Genetically Modified Polysialylated Form of Neural Cell Adhesion Molecule-Positive Cells for Potential Treatment of X-Linked Adrenoleukodystrophy
DC Field | Value | Language |
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dc.contributor.author | 강준원 | - |
dc.contributor.author | 강훈철 | - |
dc.contributor.author | 김한수 | - |
dc.contributor.author | 장지호 | - |
dc.date.accessioned | 2014-12-18T09:02:18Z | - |
dc.date.available | 2014-12-18T09:02:18Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87415 | - |
dc.description.abstract | PURPOSE: Cell transplantation of myelin-producing exogenous cells is being extensively explored as a means of remyelinating axons in X-linked adrenoleukodystrophy. We determined whether 3,3',5-Triiodo-L-thyronine (T3) overexpresses the ABCD2 gene in the polysialylated (PSA) form of neural cell adhesion molecule (NCAM)-positive cells and promotes cell proliferation and favors oligodendrocyte lineage differentiation. MATERIALS AND METHODS: PSA-NCAM+ cells from newborn Sprague-Dawley rats were grown for five days on uncoated dishes in defined medium with or without supplementation of basic fibroblast growth factor (bFGF) and/or T3. Then, PSA-NCAM+ spheres were prepared in single cells and transferred to polyornithine/fibronectin-coated glass coverslips for five days to determine the fate of the cells according to the supplementation of these molecules. T3 responsiveness of ABCD2 was analyzed using real-time quantitative polymerase chain reaction, the growth and fate of cells were determined using 5-bromo-2-deoxyuridine incorporation and immunocytochemistry, respectively. RESULTS: Results demonstrated that T3 induces overexpression of the ABCD2 gene in PSA-NCAM+ cells, and can enhance PSA-NCAM+ cell growth in the presence of bFGF, favoring an oligodendrocyte fate. CONCLUSION: These results may provide new insights into investigation of PSA-NCAM+ cells for therapeutic application to X-linked adrenoleukodystrophy. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | ATP Binding Cassette Transporter, Sub-Family D | - |
dc.subject.MESH | ATP-Binding Cassette Transporters/metabolism* | - |
dc.subject.MESH | Adrenoleukodystrophy/genetics | - |
dc.subject.MESH | Adrenoleukodystrophy/therapy* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Bromodeoxyuridine | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Fibroblast Growth Factor 2/pharmacology | - |
dc.subject.MESH | Fibronectins/metabolism | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Neural Cell Adhesion Molecules/genetics* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Sialic Acids/metabolism | - |
dc.subject.MESH | Stem Cells | - |
dc.subject.MESH | Thyroid Hormones/metabolism* | - |
dc.subject.MESH | Triiodothyronine/pharmacology | - |
dc.title | The Genetically Modified Polysialylated Form of Neural Cell Adhesion Molecule-Positive Cells for Potential Treatment of X-Linked Adrenoleukodystrophy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Jiho Jang | - |
dc.contributor.googleauthor | Han-Soo Kim | - |
dc.contributor.googleauthor | Joon Won Kang | - |
dc.contributor.googleauthor | Hoon-Chul Kang | - |
dc.identifier.doi | 10.3349/ymj.2013.54.1.246 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00082 | - |
dc.contributor.localId | A00102 | - |
dc.contributor.localId | A01100 | - |
dc.contributor.localId | A03480 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 23225827 | - |
dc.subject.keyword | ATP Binding Cassette Transporter, Sub-Family D | - |
dc.subject.keyword | ATP-Binding Cassette Transporters/metabolism* | - |
dc.subject.keyword | Adrenoleukodystrophy/genetics | - |
dc.subject.keyword | Adrenoleukodystrophy/therapy* | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Animals, Newborn | - |
dc.subject.keyword | Bromodeoxyuridine | - |
dc.subject.keyword | Cell Differentiation | - |
dc.subject.keyword | Fibroblast Growth Factor 2/pharmacology | - |
dc.subject.keyword | Fibronectins/metabolism | - |
dc.subject.keyword | Immunohistochemistry | - |
dc.subject.keyword | Neural Cell Adhesion Molecules/genetics* | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Rats, Sprague-Dawley | - |
dc.subject.keyword | Real-Time Polymerase Chain Reaction | - |
dc.subject.keyword | Sialic Acids/metabolism | - |
dc.subject.keyword | Stem Cells | - |
dc.subject.keyword | Thyroid Hormones/metabolism* | - |
dc.subject.keyword | Triiodothyronine/pharmacology | - |
dc.contributor.alternativeName | Kang, Joon Won | - |
dc.contributor.alternativeName | Kang, Hoon Chul | - |
dc.contributor.alternativeName | Kim, Han Soo | - |
dc.contributor.alternativeName | Jang, Ji Ho | - |
dc.contributor.affiliatedAuthor | Kang, Joon Won | - |
dc.contributor.affiliatedAuthor | Kang, Hoon Chul | - |
dc.contributor.affiliatedAuthor | Kim, Han Soo | - |
dc.contributor.affiliatedAuthor | Jang, Ji Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 54 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 246 | - |
dc.citation.endPage | 252 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.54(1) : 246-252, 2013 | - |
dc.identifier.rimsid | 34212 | - |
dc.type.rims | ART | - |
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