Cited 13 times in
PKA negatively regulates PP2Cβ to activate NF-κB-mediated inflammatory signaling
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최효경 | - |
dc.contributor.author | 박수연 | - |
dc.contributor.author | 윤호근 | - |
dc.date.accessioned | 2014-12-18T08:50:41Z | - |
dc.date.available | 2014-12-18T08:50:41Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87056 | - |
dc.description.abstract | Protein phosphatase 2Cβ (PP2Cβ) was found to act as a negative regulator of NF-κB-mediated inflammatory signaling; however, its regulatory mechanism has not been examined. Here, we show that protein kinase A (PKA) phosphorylates the PP2Cβ, which was inhibited by PKA-specific inhibitor, H89. Mutation analysis of serine residues in PP2Cβ revealed that Ser-195 in PP2Cβ is phosphorylated by PKA. Importantly, PKA inhibition by H89 abrogated the Forskolin-induced destabilization of PP2Cβ against ubiquitin-dependent proteosomal degradation pathway. Furthermore, H89 treatment efficiently reversed the negative effect of Forskolin on the anti-inflammatory function of PP2Cβ. Collectively, these data suggest that PKA destabilizes PP2Cβ upon inflammatory stimuli via phosphorylation of Ser-195 in PP2Cβ. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Colforsin/pharmacology | - |
dc.subject.MESH | Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors | - |
dc.subject.MESH | Cyclic AMP-Dependent Protein Kinases/metabolism* | - |
dc.subject.MESH | Enzyme Stability | - |
dc.subject.MESH | Gene Expression Regulation, Enzymologic* | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation/immunology | - |
dc.subject.MESH | Inflammation/pathology* | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins/pharmacology | - |
dc.subject.MESH | Isoquinolines/pharmacology | - |
dc.subject.MESH | NF-kappa B/immunology* | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Phosphoprotein Phosphatases/antagonists & inhibitors | - |
dc.subject.MESH | Phosphoprotein Phosphatases/immunology* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Protein Phosphatase 2C | - |
dc.subject.MESH | Proteolysis | - |
dc.subject.MESH | Serine/metabolism | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Sulfonamides/pharmacology | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/pharmacology | - |
dc.subject.MESH | Ubiquitin/metabolism | - |
dc.title | PKA negatively regulates PP2Cβ to activate NF-κB-mediated inflammatory signaling | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Hyo-Kyung Choi | - |
dc.contributor.googleauthor | Soo-Yeon Park | - |
dc.contributor.googleauthor | Hye-Jeong Oh | - |
dc.contributor.googleauthor | Eun-Jeong Han | - |
dc.contributor.googleauthor | Yoo-Hyun Lee | - |
dc.contributor.googleauthor | Jeongmin Lee | - |
dc.contributor.googleauthor | Woo Jin Jun | - |
dc.contributor.googleauthor | Kyung-Chul Choi | - |
dc.contributor.googleauthor | Ho-Geun Yoon | - |
dc.identifier.doi | 10.1016/j.bbrc.2013.05.129 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04225 | - |
dc.contributor.localId | A02625 | - |
dc.contributor.localId | A01534 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 23756813 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X13009479 | - |
dc.subject.keyword | Cytokines | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | Protein kinase A | - |
dc.subject.keyword | Protein phosphatase 2Cβ | - |
dc.subject.keyword | Ubiquitin-dependent proteasomal degradation | - |
dc.contributor.alternativeName | Choi, Hyo Kyoung | - |
dc.contributor.alternativeName | Park, Soo Yeon | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Choi, Hyo Kyoung | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Park, Soo Yeon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 436 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 473 | - |
dc.citation.endPage | 477 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.436(3) : 473-477, 2013 | - |
dc.identifier.rimsid | 32130 | - |
dc.type.rims | ART | - |
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