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PKA negatively regulates PP2Cβ to activate NF-κB-mediated inflammatory signaling

DC Field Value Language
dc.contributor.author최효경-
dc.contributor.author박수연-
dc.contributor.author윤호근-
dc.date.accessioned2014-12-18T08:50:41Z-
dc.date.available2014-12-18T08:50:41Z-
dc.date.issued2013-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/87056-
dc.description.abstractProtein phosphatase 2Cβ (PP2Cβ) was found to act as a negative regulator of NF-κB-mediated inflammatory signaling; however, its regulatory mechanism has not been examined. Here, we show that protein kinase A (PKA) phosphorylates the PP2Cβ, which was inhibited by PKA-specific inhibitor, H89. Mutation analysis of serine residues in PP2Cβ revealed that Ser-195 in PP2Cβ is phosphorylated by PKA. Importantly, PKA inhibition by H89 abrogated the Forskolin-induced destabilization of PP2Cβ against ubiquitin-dependent proteosomal degradation pathway. Furthermore, H89 treatment efficiently reversed the negative effect of Forskolin on the anti-inflammatory function of PP2Cβ. Collectively, these data suggest that PKA destabilizes PP2Cβ upon inflammatory stimuli via phosphorylation of Ser-195 in PP2Cβ.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHColforsin/pharmacology-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/antagonists & inhibitors-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/metabolism*-
dc.subject.MESHEnzyme Stability-
dc.subject.MESHGene Expression Regulation, Enzymologic*-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHumans-
dc.subject.MESHInflammation/immunology-
dc.subject.MESHInflammation/pathology*-
dc.subject.MESHIntracellular Signaling Peptides and Proteins/pharmacology-
dc.subject.MESHIsoquinolines/pharmacology-
dc.subject.MESHNF-kappa B/immunology*-
dc.subject.MESHNF-kappa B/metabolism-
dc.subject.MESHPhosphoprotein Phosphatases/antagonists & inhibitors-
dc.subject.MESHPhosphoprotein Phosphatases/immunology*-
dc.subject.MESHPhosphorylation-
dc.subject.MESHProtein Phosphatase 2C-
dc.subject.MESHProteolysis-
dc.subject.MESHSerine/metabolism-
dc.subject.MESHSignal Transduction-
dc.subject.MESHSulfonamides/pharmacology-
dc.subject.MESHTumor Necrosis Factor-alpha/pharmacology-
dc.subject.MESHUbiquitin/metabolism-
dc.titlePKA negatively regulates PP2Cβ to activate NF-κB-mediated inflammatory signaling-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorHyo-Kyung Choi-
dc.contributor.googleauthorSoo-Yeon Park-
dc.contributor.googleauthorHye-Jeong Oh-
dc.contributor.googleauthorEun-Jeong Han-
dc.contributor.googleauthorYoo-Hyun Lee-
dc.contributor.googleauthorJeongmin Lee-
dc.contributor.googleauthorWoo Jin Jun-
dc.contributor.googleauthorKyung-Chul Choi-
dc.contributor.googleauthorHo-Geun Yoon-
dc.identifier.doi10.1016/j.bbrc.2013.05.129-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04225-
dc.contributor.localIdA02625-
dc.contributor.localIdA01534-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid23756813-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X13009479-
dc.subject.keywordCytokines-
dc.subject.keywordInflammation-
dc.subject.keywordNF-κB-
dc.subject.keywordProtein kinase A-
dc.subject.keywordProtein phosphatase 2Cβ-
dc.subject.keywordUbiquitin-dependent proteasomal degradation-
dc.contributor.alternativeNameChoi, Hyo Kyoung-
dc.contributor.alternativeNamePark, Soo Yeon-
dc.contributor.alternativeNameYoon, Ho Geun-
dc.contributor.affiliatedAuthorChoi, Hyo Kyoung-
dc.contributor.affiliatedAuthorYoon, Ho Geun-
dc.contributor.affiliatedAuthorPark, Soo Yeon-
dc.rights.accessRightsnot free-
dc.citation.volume436-
dc.citation.number3-
dc.citation.startPage473-
dc.citation.endPage477-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.436(3) : 473-477, 2013-
dc.identifier.rimsid32130-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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