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Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1

Authors
 Hyang Jee  ;  Su-Hyung Lee  ;  Jun-Won Park  ;  Bo-Ram Lee  ;  Ki-Taek Nam  ;  Dae-Yong Kim 
Citation
 BMB REPORTS, Vol.46(1) : 25-30, 2013 
Journal Title
 BMB REPORTS 
ISSN
 1976-6696 
Issue Date
2013
MeSH
Cell Line, Tumor ; Cell Proliferation ; Connexins/genetics ; Connexins/metabolism* ; Cyclin-Dependent Kinase Inhibitor p21/genetics ; Cyclin-Dependent Kinase Inhibitor p21/metabolism* ; Cyclin-Dependent Kinase Inhibitor p27/genetics ; Cyclin-Dependent Kinase Inhibitor p27/metabolism* ; G1 Phase Cell Cycle Checkpoints ; Gene Expression Regulation, Neoplastic* ; Humans ; Immunohistochemistry ; Ki-67 Antigen/genetics ; Ki-67 Antigen/metabolism ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; Transfection ; Up-Regulation
Keywords
AGS cells ; Cell cycle distribution ; Cell proliferation ; Connexin32 ; Gastric cancer
Abstract
Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21Cip1 and p27Kip1 expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21Cip1 and p27Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins.
Full Text
http://www.bmbreports.org/fulltext/bmbreports/view.php?vol=46&page=25
DOI
23351380
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86988
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