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Gallic acid, a natural polyphenolic acid, induces apoptosis and inhibits proin?ammatory gene expressions in rheumatoid arthritis ?broblast-like synoviocytes

DC FieldValueLanguage
dc.contributor.author박민찬-
dc.contributor.author박용범-
dc.contributor.author이상원-
dc.contributor.author이수곤-
dc.date.accessioned2014-12-18T08:47:35Z-
dc.date.available2014-12-18T08:47:35Z-
dc.date.issued2013-
dc.identifier.issn1297-319X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86962-
dc.description.abstractOBJECTIVES: To investigate whether gallic acid (3, 4, 5-trihydroxybenzoic acid), a natural polyphenolic acid found in gall nuts, sumac, oak bark, tea leaves, grapes and wine, has pro-apoptotic and anti-inflammatory effects on fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). METHODS: Viability of RA FLS was assessed using a MTT assay after gallic acid treatment. Apoptosis was assessed by TUNEL assay and caspase-3 activity was determined by a colorimetric assay. The levels of apoptosis-related proteins including Bcl-2, p-Akt, p53, and Bax were determined using western blot analyses, and the mRNA expressions of various pro-inflammatory mediators were measured using quantitative real-time PCR. RESULTS: Cell viability of RA FLS was significantly decreased by treatment with 10 or more μM of gallic acid. Gallic acid treatment at the concentrations that do not affect cell viability (0.1 and 1 μM) induced cellular apoptosis of RA FLS. Treatment with 0.1 and 1 μM of gallic acid also resulted in a significant increase in caspase-3 activity and regulated the productions of Bcl-2, Bax, p53 and pAkt. The mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6), chemokines (CCL-2/MCP-1, CCL-7/MCP-3), cyclooxygenase-2, and matrix metalloproteinase-9 from RA FLS were suppressed by the gallic acid treatment in dose-dependent manners. CONCLUSION: Gallic acid treatment was found to induce apoptosis of RA FLS through regulation of apoptosis-related protein expressions and to reduce the expression of pro-inflammatory genes in RA FLS. These data suggest that pro-apoptotic and anti-inflammatory activities of gallic acid may be used as a possible therapeutic option for RA.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOINT BONE SPINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHArthritis, Rheumatoid/drug therapy*-
dc.subject.MESHArthritis, Rheumatoid/genetics-
dc.subject.MESHArthritis, Rheumatoid/immunology-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHCells, Cultured-
dc.subject.MESHFibroblasts/cytology-
dc.subject.MESHFibroblasts/drug effects*-
dc.subject.MESHFibroblasts/physiology-
dc.subject.MESHGallic Acid/pharmacology*-
dc.subject.MESHGene Expression Regulation/drug effects*-
dc.subject.MESHGene Expression Regulation/immunology-
dc.subject.MESHHumans-
dc.subject.MESHIn Situ Nick-End Labeling-
dc.subject.MESHPolyphenols/pharmacology-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHSynovial Membrane/cytology-
dc.subject.MESHSynovial Membrane/drug effects*-
dc.subject.MESHSynovial Membrane/physiology-
dc.titleGallic acid, a natural polyphenolic acid, induces apoptosis and inhibits proin?ammatory gene expressions in rheumatoid arthritis ?broblast-like synoviocytes-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorChong-Hyeon Yoon-
dc.contributor.googleauthorSoo-Jin Chung-
dc.contributor.googleauthorSang-Won Lee-
dc.contributor.googleauthorYong-Beom Park-
dc.contributor.googleauthorSoo-Kon Lee-
dc.contributor.googleauthorMin-Chan Park-
dc.identifier.doi10.1016/j.jbspin.2012.08.010-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01470-
dc.contributor.localIdA01579-
dc.contributor.localIdA02889-
dc.contributor.localIdA03638-
dc.contributor.localIdA02824-
dc.relation.journalcodeJ01216-
dc.identifier.eissn1778-7254-
dc.identifier.pmid23058179-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1297319X1200200X-
dc.subject.keywordRheumatoid arthritis-
dc.subject.keywordFibroblast-like synoviocytes-
dc.subject.keywordGallic acid-
dc.subject.keywordApoptosis-
dc.subject.keywordInflammation-
dc.contributor.alternativeNamePark, Min Chan-
dc.contributor.alternativeNamePark, Yong Beom-
dc.contributor.alternativeNameLee, Sang Won-
dc.contributor.alternativeNameLee, Soo Kon-
dc.contributor.alternativeNameJeong, Su Jin-
dc.contributor.affiliatedAuthorPark, Min Chan-
dc.contributor.affiliatedAuthorPark, Yong Beom-
dc.contributor.affiliatedAuthorLee, Soo Kon-
dc.contributor.affiliatedAuthorJeong, Su Jin-
dc.contributor.affiliatedAuthorLee, Sang Won-
dc.rights.accessRightsnot free-
dc.citation.volume80-
dc.citation.number3-
dc.citation.startPage274-
dc.citation.endPage279-
dc.identifier.bibliographicCitationJOINT BONE SPINE, Vol.80(3) : 274-279, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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