미세생리시스템을 사용한 인체 골격근 모델링

Abstract

Purpose: Conventional 2-dimensional cultures and animal models have limited ability to reproduce the structural complexity, dynamic mechanical cues, and sustained functionality of native skeletal muscle tissue. To overcome these limitations, skeletal muscle-on-a-chip platforms have been developed as advanced in vitro systems for studying muscle physiology, pathology, and regeneration. Current Concepts: These microengineered systems incorporate essential features of skeletal muscle, including 3-dimensional architecture, cellular alignment, contractile function, and responsiveness to biochemical and mechanical stimuli. Recent advances, such as vascularization, multi-organ integration, and spaceflight-compatible designs, have expanded their applications in disease modeling and drug screening. Discussion and Conclusion: This review examines key engineering strategies, biological performance metrics, and representative applications of skeletal muscle-on-a-chip systems. It also addresses technical challenges, including long-term functionality, measurement standardization, and clinical translation, and considers future prospects for their integration into preclinical testing and regenerative medicine.