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In vivo tracking of transfused platelets by next-generation sequencing of mitochondrial DNA

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dc.contributor.author권순성-
dc.contributor.author김신영-
dc.contributor.author정준원-
dc.contributor.author정해림-
dc.contributor.author조현수-
dc.contributor.author최승준-
dc.date.accessioned2025-10-15T01:44:08Z-
dc.date.available2025-10-15T01:44:08Z-
dc.date.issued2025-09-
dc.identifier.issn2473-9529-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/207451-
dc.description.statementOfResponsibilityT202506017.pdf-
dc.languageEnglish-
dc.publisherAmerican Society of Hematology-
dc.relation.isPartOfBLOOD ADVANCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESH0-
dc.titleIn vivo tracking of transfused platelets by next-generation sequencing of mitochondrial DNA-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorSeung Jun Choi-
dc.contributor.googleauthorSoon Sung Kwon-
dc.contributor.googleauthorHyun Kyung Kim-
dc.contributor.googleauthorEun Jung Suh-
dc.contributor.googleauthorHaerim Chung-
dc.contributor.googleauthorHyunsoo Cho-
dc.contributor.googleauthorJune-Won Cheong-
dc.contributor.googleauthorSinyoung Kim-
dc.identifier.doiTracking transfused platelets is important to evaluate platelet transfusion efficiency. Traditionally, corrected count increments were used; however, quantitative polymerase chain reaction-based methods have recently been developed. As both these methods have some limitations, we developed a new method based on next-generation sequencing (NGS) of platelet mitochondrial DNA (mtDNA). We identified several single nucleotide variant markers by sequencing the entire mtDNA region of platelets, and used NGS to estimate the proportion of each platelet unit. This method was validated using mixed platelets obtained from different donors at various ratios. We confirmed the applicability of this method in patients who received platelet transfusions using pre- and posttransfusion samples from 12 patients with hematological malignancies. The method showed good linearity (r2 >0.99 in the range of mixing ratios from 1:1 to 1:50) in the platelet-mixing experiment. In addition, platelet tracking in patients who received transfusions was feasible using this method. Furthermore, it was possible to track individual platelets in patients who received a single platelet transfusion and in those who received multiple transfusions, including a patient who received 5 platelet transfusions. Hence, this NGS-based platelet-tracking method can be used for patients with various conditions.-
dc.contributor.localIdA06015-
dc.contributor.localIdA00675-
dc.contributor.localIdA03729-
dc.contributor.localIdA04674-
dc.contributor.localIdA03929-
dc.contributor.localIdA05522-
dc.relation.journalcodeJ04280-
dc.identifier.eissn2473-9537-
dc.identifier.pmid40554360-
dc.subject.keywordBlood Component Removal-
dc.subject.keywordBlood Donors-
dc.subject.keywordBlood Platelets* / cytology-
dc.subject.keywordBlood Platelets* / metabolism-
dc.subject.keywordCell Separation* / methods-
dc.subject.keywordDNA, Mitochondrial* / analysis-
dc.subject.keywordDNA, Mitochondrial* / genetics-
dc.subject.keywordDNA, Mitochondrial* / metabolism-
dc.subject.keywordFeasibility Studies-
dc.subject.keywordHematologic Neoplasms / therapy-
dc.subject.keywordHigh-Throughput Nucleotide Sequencing-
dc.subject.keywordHumans-
dc.subject.keywordPlatelet Transfusion*-
dc.subject.keywordPolymorphism, Single Nucleotide-
dc.subject.keywordTreatment Outcome-
dc.contributor.alternativeNameKwon, Sun Sung-
dc.contributor.affiliatedAuthor권순성-
dc.contributor.affiliatedAuthor김신영-
dc.contributor.affiliatedAuthor정준원-
dc.contributor.affiliatedAuthor정해림-
dc.contributor.affiliatedAuthor조현수-
dc.contributor.affiliatedAuthor최승준-
dc.citation.volume9-
dc.citation.number17-
dc.citation.startPage4357-
dc.citation.endPage4363-
dc.identifier.bibliographicCitationBLOOD ADVANCES, Vol.9(17) : 4357-4363, 2025-09-
dc.identifier.articleno10.1182/bloodadvances.2024015009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
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