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Comparative analysis of salivary cortisol measurements using different assay methods in relation to serum-free cortisol measurement
DC Field | Value | Language |
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dc.contributor.author | 석정호 | - |
dc.date.accessioned | 2024-07-18T05:20:42Z | - |
dc.date.available | 2024-07-18T05:20:42Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/200067 | - |
dc.description.abstract | Objectives: Salivary cortisol reflects the biologically active form of serum cortisol, offering a noninvasive evaluation method for the diurnal rhythm of the hypothalamic-pituitary-adrenal (HPA) axis. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) is known for its specificity, immunoassays (IA) are commonly used because of their simplicity. This study aimed to assess the performance of salivary cortisol measurement using both IA and LC-MS/MS in comparison to serum-free cortisol measurement. Methods: Assay results for 188 saliva and 94 serum samples from 47 participants were analyzed. Salivary samples collected at different time points were analyzed using IA and LC-MS/MS. Serum samples were analyzed for cortisol, cortisol-binding globulin, and free cortisol. The statistical analyses included correlations and method comparisons. Results: The diurnal salivary cortisol profiles exhibited a comparable circadian rhythm pattern; however, the concentrations measured using IA were consistently higher than those measured using LC-MS/MS. The correlation analysis revealed robust associations among salivary cortisol (IA), salivary cortisol (LC-MS/MS), and serum-free cortisol levels (LC-MS/MS). However, the method comparison revealed a systematic bias between IA and LC-MS/MS in salivary cortisol measurement. Conclusions: This study contributes to the ongoing debate on assay techniques by affirming the suitability of IA and LC-MS/MS for salivary cortisol measurement to assess dynamic changes in HPA axis activity. The identified systematic bias emphasizes the importance of selecting methods based on specific research or clinical requirements. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isPartOf | Practical Laboratory Medicine | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Comparative analysis of salivary cortisol measurements using different assay methods in relation to serum-free cortisol measurement | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Psychiatry (정신과학교실) | - |
dc.contributor.googleauthor | Anna Lee | - |
dc.contributor.googleauthor | Sooah Jang | - |
dc.contributor.googleauthor | Sanghoo Lee | - |
dc.contributor.googleauthor | Hyun-Kyung Park | - |
dc.contributor.googleauthor | In-Young Kim | - |
dc.contributor.googleauthor | Ryunsup Ahn | - |
dc.contributor.googleauthor | Jeong-Ho Seok | - |
dc.contributor.googleauthor | Kyoung-Ryul Lee | - |
dc.identifier.doi | 10.1016/j.plabm.2024.e00393 | - |
dc.contributor.localId | A01929 | - |
dc.relation.journalcode | J03550 | - |
dc.identifier.eissn | 2352-5517 | - |
dc.identifier.pmid | 38645932 | - |
dc.subject.keyword | Diurnal rhythm | - |
dc.subject.keyword | Hypothalamic-pituitary-adrenal axis | - |
dc.subject.keyword | Immunoassay | - |
dc.subject.keyword | Liquid chromatography-tandem mass spectrometry | - |
dc.subject.keyword | Salivary cortisol | - |
dc.contributor.alternativeName | Seok, Jeong Ho | - |
dc.contributor.affiliatedAuthor | 석정호 | - |
dc.citation.volume | 40 | - |
dc.citation.startPage | e00393 | - |
dc.identifier.bibliographicCitation | Practical Laboratory Medicine, Vol.40 : e00393, 2024-04 | - |
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