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Soluble Sema4D cleaved from osteoclast precursors by TACE suppresses osteoblastogenesis
DC Field | Value | Language |
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dc.contributor.author | 김재영 | - |
dc.date.accessioned | 2024-05-30T07:03:21Z | - |
dc.date.available | 2024-05-30T07:03:21Z | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 1582-1838 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/199542 | - |
dc.description.abstract | Bone remodelling is mediated by orchestrated communication between osteoclasts and osteoblasts which, in part, is regulated by coupling and anti-coupling factors. Amongst formally known anti-coupling factors, Semaphorin 4D (Sema4D), produced by osteoclasts, plays a key role in downmodulating osteoblastogenesis. Sema4D is produced in both membrane-bound and soluble forms; however, the mechanism responsible for producing sSema4D from osteoclasts is unknown. Sema4D, TACE and MT1-MMP are all expressed on the surface of RANKL-primed osteoclast precursors. However, only Sema4D and TACE were colocalized, not Sema4D and MT1-MMP. When TACE and MT1-MMP were either chemically inhibited or suppressed by siRNA, TACE was found to be more engaged in shedding Sema4D. Anti-TACE-mAb inhibited sSema4D release from osteoclast precursors by similar to 90%. Supernatant collected from osteoclast precursors (OC-sup) suppressed osteoblastogenesis from MC3T3-E1 cells, as measured by alkaline phosphatase activity, but OC-sup harvested from the osteoclast precursors treated with anti-TACE-mAb restored osteoblastogenesis activity in a manner that compensates for diminished sSema4D. Finally, systemic administration of anti-TACE-mAb downregulated the generation of sSema4D in the mouse model of critical-sized bone defect, whereas local injection of recombinant sSema4D to anti-TACE-mAb-treated defect upregulated local osteoblastogenesis. Therefore, a novel pathway is proposed whereby TACE-mediated shedding of Sema4D expressed on the osteoclast precursors generates functionally active sSema4D to suppress osteoblastogenesis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Matrix Metalloproteinase 14 / metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Osteoblasts / metabolism | - |
dc.subject.MESH | Osteoclasts* / metabolism | - |
dc.subject.MESH | Semaphorins* / genetics | - |
dc.subject.MESH | Semaphorins* / metabolism | - |
dc.title | Soluble Sema4D cleaved from osteoclast precursors by TACE suppresses osteoblastogenesis | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Prosthodontics (보철과학교실) | - |
dc.contributor.googleauthor | Takenobu Ishii | - |
dc.contributor.googleauthor | Montserrat Ruiz-Torruella | - |
dc.contributor.googleauthor | Jae Young Kim | - |
dc.contributor.googleauthor | Hiroyuki Kanzaki | - |
dc.contributor.googleauthor | Abdullah Albassam | - |
dc.contributor.googleauthor | Wichaya Wisitrasameewong | - |
dc.contributor.googleauthor | Satoru Shindo | - |
dc.contributor.googleauthor | Roodelyne Pierrelus | - |
dc.contributor.googleauthor | Alireza Heidari | - |
dc.contributor.googleauthor | Umadevi Kandalam | - |
dc.contributor.googleauthor | Shin Nakamura | - |
dc.contributor.googleauthor | Alexandru Movila | - |
dc.contributor.googleauthor | Dmitriy Minond | - |
dc.contributor.googleauthor | Toshihisa Kawai | - |
dc.identifier.doi | 10.1111/jcmm.17416 | - |
dc.contributor.localId | A06406 | - |
dc.relation.journalcode | J01302 | - |
dc.identifier.eissn | 1582-4934 | - |
dc.identifier.pmid | 37170687 | - |
dc.subject.keyword | membrane type-1 matrix metalloproteinase | - |
dc.subject.keyword | osteoblastogenesis | - |
dc.subject.keyword | osteoclasts | - |
dc.subject.keyword | semaphorin 4D | - |
dc.subject.keyword | sheddase | - |
dc.subject.keyword | tumor necrosis factor alpha converting enzyme | - |
dc.contributor.alternativeName | Kim, Jae-Young | - |
dc.contributor.affiliatedAuthor | 김재영 | - |
dc.citation.volume | 27 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1750 | - |
dc.citation.endPage | 1756 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol.27(12) : 1750-1756, 2023-06 | - |
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