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Comparison of Glioblastoma Cell Culture Platforms Based on Transcriptional Similarity with Paired Tissue

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dc.contributor.author강석구-
dc.contributor.author김의현-
dc.contributor.author문주형-
dc.contributor.author장종희-
dc.contributor.author박준성-
dc.contributor.author오유정-
dc.contributor.author심진경-
dc.date.accessioned2024-05-23T03:34:50Z-
dc.date.available2024-05-23T03:34:50Z-
dc.date.issued2024-04-
dc.identifier.issn1424-8247-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/199259-
dc.description.abstractNo standardized in vitro cell culture models for glioblastoma (GBM) have yet been estab- lished, excluding the traditional two-dimensional culture. GBM tumorspheres (TSs) have been high-lighted as a good model platform for testing drug effects and characterizing specific features of GBM, but a detailed evaluation of their suitability and comparative performance is lacking. Here, we isolated GBM TSs and extracellular matrices (ECM) from tissues obtained from newly diagnosed IDH1 wild-type GBM patients and cultured GBM TSs on five different culture platforms: (1) ordinary TS culture liquid media (LM), (2) collagen-based three-dimensional (3D) matrix, (3) patient typical ECM-based 3D matrix, (4) patient tumor ECM-based 3D matrix, and (5) mouse brain. For evaluation, we obtained transcriptome data from all cultured GBM TSs using microarrays. The LM platform exhibited the most similar transcriptional program to paired tissues based on GBM genes, stemness- and invasiveness-related genes, transcription factor activity, and canonical signaling pathways. GBM TSs can be cultured via an easy-to-handle and cost- and time-efficient LM platform while preserving the transcriptional program of the originating tissues without supplementing the ECM or embedding it into the mouse brain. In addition to applications in basic cancer research, GBM TSs cultured in LM may also serve as patient avatars in drug screening and pre-clinical evaluation of targeted therapy and as standardized and clinically relevant models for precision medicine.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfPHARMACEUTICALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleComparison of Glioblastoma Cell Culture Platforms Based on Transcriptional Similarity with Paired Tissue-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Neurosurgery (신경외과학교실)-
dc.contributor.googleauthorJunseong Park-
dc.contributor.googleauthorIlkyoo Koh-
dc.contributor.googleauthorJunghwa Cha-
dc.contributor.googleauthorYoojung Oh-
dc.contributor.googleauthorJin-Kyoung Shim-
dc.contributor.googleauthorHyejin Kim-
dc.contributor.googleauthorJu Hyung Moon-
dc.contributor.googleauthorEui Hyun Kim-
dc.contributor.googleauthorJong Hee Chang-
dc.contributor.googleauthorPilnam Kim-
dc.contributor.googleauthorSeok-Gu Kang-
dc.identifier.doi10.3390/ph17040529-
dc.contributor.localIdA00036-
dc.contributor.localIdA00837-
dc.contributor.localIdA01383-
dc.contributor.localIdA03470-
dc.relation.journalcodeJ04088-
dc.identifier.pmid38675489-
dc.subject.keywordcell culture platform-
dc.subject.keywordextracellular matrix-
dc.subject.keywordglioblastoma-
dc.subject.keywordpatient-derived tumorsphere-
dc.subject.keywordtranscriptional program-
dc.contributor.alternativeNameKang, Seok Gu-
dc.contributor.affiliatedAuthor강석구-
dc.contributor.affiliatedAuthor김의현-
dc.contributor.affiliatedAuthor문주형-
dc.contributor.affiliatedAuthor장종희-
dc.citation.volume17-
dc.citation.number4-
dc.citation.startPage529-
dc.identifier.bibliographicCitationPHARMACEUTICALS, Vol.17(4) : 529, 2024-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers

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