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Protocol for cross-platform characterization of human and murine extracellular vesicles and particles

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dc.contributor.authorBojmar, Linda-
dc.contributor.authorKim, Han Sang-
dc.contributor.authorSugiura, Kei-
dc.contributor.authorHeissel, Soren-
dc.contributor.authorLucotti, Serena-
dc.contributor.authorCioffi, Michele-
dc.contributor.authorJohnson, Kofi Ennu-
dc.contributor.authorCohen-Gould, Leona-
dc.contributor.authorZhang, Haiying-
dc.contributor.authorMolina, Henrik-
dc.contributor.authorMatei, Irina R.-
dc.contributor.authorLyden, David-
dc.contributor.authorHoshino, Ayuko-
dc.date.accessioned2024-04-11T06:22:59Z-
dc.date.available2024-04-11T06:22:59Z-
dc.date.created2024-04-19-
dc.date.issued2024-03-
dc.identifier.issn2666-1667-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/198784-
dc.description.abstractCharacterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross -validation across different platforms.For complete details on the use and execution of this protocol, please refer to Hoshino et al.1-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherCell Press-
dc.relation.isPartOfSTAR PROTOCOLS-
dc.relation.isPartOfSTAR Protocols-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleProtocol for cross-platform characterization of human and murine extracellular vesicles and particles-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorBojmar, Linda-
dc.contributor.googleauthorKim, Han Sang-
dc.contributor.googleauthorSugiura, Kei-
dc.contributor.googleauthorHeissel, Soren-
dc.contributor.googleauthorLucotti, Serena-
dc.contributor.googleauthorCioffi, Michele-
dc.contributor.googleauthorJohnson, Kofi Ennu-
dc.contributor.googleauthorCohen-Gould, Leona-
dc.contributor.googleauthorZhang, Haiying-
dc.contributor.googleauthorMolina, Henrik-
dc.contributor.googleauthorMatei, Irina R.-
dc.contributor.googleauthorLyden, David-
dc.contributor.googleauthorHoshino, Ayuko-
dc.identifier.doi10.1016/j.xpro.2023.102754-
dc.relation.journalcodeJ04093-
dc.identifier.pmid38096060-
dc.subject.keywordCell Biology-
dc.subject.keywordCell isolation-
dc.subject.keywordCell Membrane-
dc.subject.keywordMass Spectrometry-
dc.subject.keywordModel Organisms-
dc.subject.keywordMolecular Biology-
dc.subject.keywordProtein Biochemistry-
dc.subject.keywordProteomics-
dc.contributor.alternativeNameKim, Han Sang-
dc.contributor.affiliatedAuthorKim, Han Sang-
dc.identifier.scopusid2-s2.0-85180007425-
dc.identifier.wosid001140945300001-
dc.citation.volume5-
dc.citation.number1-
dc.identifier.bibliographicCitationSTAR PROTOCOLS, Vol.5(1), 2024-03-
dc.identifier.rimsid83495-
dc.type.rimsART-
dc.description.journalClass1-
dc.description.journalClass1-
dc.subject.keywordAuthorCell Biology-
dc.subject.keywordAuthorCell isolation-
dc.subject.keywordAuthorCell Membrane-
dc.subject.keywordAuthorMass Spectrometry-
dc.subject.keywordAuthorModel Organisms-
dc.subject.keywordAuthorMolecular Biology-
dc.subject.keywordAuthorProtein Biochemistry-
dc.subject.keywordAuthorProteomics-
dc.subject.keywordPlusSAMPLE PREPARATION METHOD-
dc.subject.keywordPlusFLOW CYTOMETER-
dc.type.docTypeArticle-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.identifier.articleno102754-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
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