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Identification of dendritic cell precursor from the CD11c+ cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand
DC Field | Value | Language |
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dc.contributor.author | 김태균 | - |
dc.contributor.author | 나혜영 | - |
dc.contributor.author | 박채규 | - |
dc.contributor.author | 주민경 | - |
dc.contributor.author | 박제연 | - |
dc.date.accessioned | 2024-01-16T01:56:38Z | - |
dc.date.available | 2024-01-16T01:56:38Z | - |
dc.date.issued | 2023-11 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/197793 | - |
dc.description.abstract | Dendritic cells (DCs) are readily generated from the culture of mouse bone marrow (BM) treated with either granulocyte macrophage-colony stimulating factor (GM-CSF) or FMS-like tyrosine kinase 3 ligand (FLT3L). CD11c+MHCII+ or CD11c+MHCIIhi cells are routinely isolated from those BM cultures and generally used as in vitro-generated DCs for a variety of experiments and therapies. Here, we examined CD11c+ cells in the BM culture with GM-CSF or FLT3L by staining with a monoclonal antibody 2A1 that is known to recognize mature or activated DCs. Most of the cells within the CD11c+MHCIIhi DC gate were 2A1+ in the BM culture with GM-CSF (GM-BM culture). In the BM culture with FLT3L (FL-BM culture), almost of all the CD11c+MHCIIhi cells were within the classical DC2 (cDC2) gate. The analysis of FL-BM culture revealed that a majority of cDC2-gated CD11c+MHCIIhi cells exhibited a 2A1-CD83-CD115+CX3CR1+ phenotype, and the others consisted of 2A1+CD83+CD115-CX3CR1- and 2A1-CD83-CD115-CX3CR1- cells. According to the antigen uptake and presentation, morphologies, and gene expression profiles, 2A1-CD83-CD115-CX3CR1- cells were immature cDC2s and 2A1+CD83+CD115-CX3CR1- cells were mature cDC2s. Unexpectedly, however, 2A1-CD83-CD115+CX3CR1+ cells, the most abundant cDC2-gated MHCIIhi cell subset in FL-BM culture, were non-DCs. Adoptive cell transfer experiments in the FL-BM culture confirmed that the cDC2-gated MHCIIhi non-DCs were precursors to cDC2s, i.e., MHCIIhi pre-cDC2s. MHCIIhi pre-cDC2s also expressed the higher level of DC-specific transcription factor Zbtb46 as similarly as immature cDC2s. Besides, MHCIIhi pre-cDC2s were generated only from pre-cDCs and common DC progenitor (CDP) cells but not from monocytes and common monocyte progenitor (cMoP) cells, verifying that MHCIIhi pre-cDC2s are close lineage to cDCs. All in all, our study identified and characterized a new cDC precursor, exhibiting a CD11c+MHCIIhiCD115+CX3CR1+ phenotype, in FL-BM culture. Copyright © 2023 In, Park, Shin, Ryu, Sohn, Choi, Park, Hwang, Park, Che, Kim, Chu, Na and Park. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Frontiers Research Foundation | - |
dc.relation.isPartOf | FRONTIERS IN IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bone Marrow Cells | - |
dc.subject.MESH | Bone Marrow* | - |
dc.subject.MESH | CX3C Chemokine Receptor 1 / metabolism | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Dendritic Cells | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor / metabolism | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology | - |
dc.subject.MESH | Histocompatibility Antigens Class II* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Receptor Protein-Tyrosine Kinases / metabolism | - |
dc.title | Identification of dendritic cell precursor from the CD11c+ cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.googleauthor | Hyunju In | - |
dc.contributor.googleauthor | Ji Soo Park | - |
dc.contributor.googleauthor | Hyun Soo Shin | - |
dc.contributor.googleauthor | Seul Hye Ryu | - |
dc.contributor.googleauthor | Moah Sohn | - |
dc.contributor.googleauthor | Wanho Choi | - |
dc.contributor.googleauthor | Sejung Park | - |
dc.contributor.googleauthor | Soomin Hwang | - |
dc.contributor.googleauthor | Jeyun Park | - |
dc.contributor.googleauthor | Lihua Che | - |
dc.contributor.googleauthor | Tae-Gyun Kim | - |
dc.contributor.googleauthor | Min Kyung Chu | - |
dc.contributor.googleauthor | Hye Young Na | - |
dc.contributor.googleauthor | Chae Gyu Park | - |
dc.identifier.doi | 10.3389/fimmu.2023.1179981 | - |
dc.contributor.localId | A05324 | - |
dc.contributor.localId | A04556 | - |
dc.contributor.localId | A01718 | - |
dc.contributor.localId | A03950 | - |
dc.relation.journalcode | J03075 | - |
dc.identifier.eissn | 1664-3224 | - |
dc.identifier.pmid | 38094300 | - |
dc.subject.keyword | FLT3 ligand | - |
dc.subject.keyword | GM-CSF | - |
dc.subject.keyword | antigen presentation | - |
dc.subject.keyword | bone marrow cells | - |
dc.subject.keyword | cell differentiation | - |
dc.subject.keyword | cultured cells | - |
dc.subject.keyword | dendritic cells | - |
dc.subject.keyword | precursor cells | - |
dc.contributor.alternativeName | Kim, Tae-Gyun | - |
dc.contributor.affiliatedAuthor | 김태균 | - |
dc.contributor.affiliatedAuthor | 나혜영 | - |
dc.contributor.affiliatedAuthor | 박채규 | - |
dc.contributor.affiliatedAuthor | 주민경 | - |
dc.citation.volume | 14 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN IMMUNOLOGY, Vol.14, 2023-11 | - |
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