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Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation

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dc.contributor.author양재석-
dc.contributor.author엄희정-
dc.date.accessioned2023-11-07T07:51:13Z-
dc.date.available2023-11-07T07:51:13Z-
dc.date.issued2023-10-
dc.identifier.issn0022-1759-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/196538-
dc.description.abstractTransplantation of organs, cells, or tissues from one species to another, known as xenotransplantation, has the potential to alleviate organ donor shortages and enhance the success of organ transplantation. However, the possibility of immunological rejection by the recipient is one of the biggest difficulties associated with xenotransplantation. The creation of neutrophil extracellular traps (NETs), also known as NETosis, is hypothesized as a mechanism of rejection. Innovations in microfluidics and co-culturing techniques have provided access to several classes of microengineered model systems in experimental models, connecting animal research and traditional in vitro methods such as organoids, microphysiological systems, and organs-on-chip. To achieve this goal, we established a perfusable 3D Xeno vessel chip using a porcine aortic endothelial cell line and examined how NETs grow when isolated human and primate neutrophils were used. Neutrophils from both humans and monkeys displayed the usual NETosis phases, including nuclear decondensation, enlargement, and rounding of DNA, occupying the entire cytoplasm, and discharge of fragmented DNA after cell membrane rupture. Using confocal fluorescence imaging of DNA and citrullinated histone colocalization and DNA histone complex formation in supernatants from xeno vessel chips, we confirmed NETs generation by human and monkey neutrophils when cocultured in a xeno-vessel chip.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfJOURNAL OF IMMUNOLOGICAL METHODS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHExtracellular Traps*-
dc.subject.MESHHistones-
dc.subject.MESHHumans-
dc.subject.MESHNeutrophils-
dc.subject.MESHOrgan Transplantation*-
dc.subject.MESHSwine-
dc.subject.MESHTransplantation, Heterologous-
dc.titleApplication of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorSuchen Kumar Yadav-
dc.contributor.googleauthorSeokwoo Park-
dc.contributor.googleauthorYun-Mi Lee-
dc.contributor.googleauthorSunghoon Hurh-
dc.contributor.googleauthorDongsuk Kim-
dc.contributor.googleauthorSangil Min-
dc.contributor.googleauthorSejoong Kim-
dc.contributor.googleauthorJi-Jing Yan-
dc.contributor.googleauthorByeong-Cheol Kang-
dc.contributor.googleauthorSungjoo Kim-
dc.contributor.googleauthorJaeseok Yang-
dc.contributor.googleauthorJong Cheol Jeong-
dc.identifier.doi10.1016/j.jim.2023.113537-
dc.contributor.localIdA06130-
dc.relation.journalcodeJ01449-
dc.identifier.eissn1872-7905-
dc.identifier.pmid37598787-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0022175923001199-
dc.subject.keywordDNA-histone complex-
dc.subject.keywordMicrofluidics-
dc.subject.keywordNeutrophil extracellular traps (NETs)-
dc.subject.keywordNeutrophils-
dc.subject.keywordXeno vessel–chip-
dc.subject.keywordXenotransplantation-
dc.contributor.alternativeNameYang, Jaeseok-
dc.contributor.affiliatedAuthor양재석-
dc.citation.volume521-
dc.citation.startPage113537-
dc.identifier.bibliographicCitationJOURNAL OF IMMUNOLOGICAL METHODS, Vol.521 : 113537, 2023-10-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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