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Optimization of the optical transparency of bones by PACT-based passive tissue clearing

Authors
 Byung-Ho Jin  ;  Jiwon Woo  ;  Mirae Lee  ;  Seockmo Ku  ;  Hyung Seok Moon  ;  Seung Jun Ryu  ;  Young-Min Hyun  ;  Jeong-Yoon Park  ;  Sung Uk Kuh  ;  Yong Eun Cho 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE : epub, 2023-10 
Journal Title
EXPERIMENTAL AND MOLECULAR MEDICINE
ISSN
 1226-3613 
Issue Date
2023-10
Abstract
Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and biological circuits. Nonetheless, the issues that remain in maintaining structural integrity and preventing tissue expansion/shrinkage with rapid clearing still inhibit the wide application of these techniques in hard bone tissues, such as femurs and tibias. Here, we present an optimized PACT-based bone-clearing method, Bone-mPACT+, that protects biological structures. Bone-mPACT+ and four different decalcifying procedures were tested for their ability to improve bone tissue clearing efficiency without sacrificing optical transparency; they rendered nearly all types of bone tissues transparent. Both mouse and rat bones were nearly transparent after the clearing process. We also present a further modification, the Bone-mPACT+ Advance protocol, which is specifically optimized for processing the largest and hardest rat bones for easy clearing and imaging using established tissue clearing methods.

© 2023. The Author(s).
Files in This Item:
T202305672.pdf Download
DOI
10.1038/s12276-023-01089-8
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kuh, Sung Uk(구성욱) ORCID logo https://orcid.org/0000-0003-2566-3209
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
Woo, Jiwon(우지원)
Cho, Yong Eun(조용은) ORCID logo https://orcid.org/0000-0001-9815-2720
Jin, Byung Ho(진병호)
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/196523
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