Cited 15 times in
Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway
DC Field | Value | Language |
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dc.date.accessioned | 2023-08-09T02:49:41Z | - |
dc.date.available | 2023-08-09T02:49:41Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195873 | - |
dc.description.abstract | Dysregulation of collagen production contributes to various pathological processes, including tissue fibrosis as well as impaired wound healing. Lipo-prostaglandin E1 (Lipo-PGE1), a lipid microsphere-incorporated prostaglandin E1, is used as a vasodilator for the treatment of peripheral vascular diseases. Lipo-PGE1 was recently shown to enhance human dermal fibroblast (HDF) migration and in vivo wound healing. No published study has characterized the role of Lipo-PGE1 in collagen regulation in HDFs. Here, we investigated the cellular signaling mechanism by which Lipo-PGE1 regulates collagen in HDFs. Collagen production was evaluated by the Sircol collagen assay, Western blot analysis of type I collagen and real time PCR. Unexpectedly, Lipo-PGE1 decreased mRNA expression of collagen 1A1, 1A2, and 3A1. Lipo-PGE1 markedly inhibited type I collagen and total soluble collagen production. In addition, Lipo-PGE1 inhibited transforming growth factor-β-induced collagen expression via Smad2 phosphorylation. To further investigate whether extracellular signal-regulated kinase (ERK)/Ets-1 signaling, a crucial pathway in collagen regulation, is involved in Lipo-PGE1-inhibited collagen production, cells were pretreated with an ERK-specific inhibitor, PD98059, prior to the addition of Lipo-PGE1. Lipo-PGE1-inhibited collagen mRNA expression and total soluble collagen production were recovered by pretreatment with PD98059. Moreover, Lipo-PGE1 directly induced the phosphorylation of ERK. Furthermore, silencing of Ets-1 recovered Lipo-PGE1-inhibited collagen production and PD98059 blocked Lipo-PGE1-enhanced Ets-1 expression. The present study reveals an important role for Lipo-PGE1 as a negative regulator of collagen gene expression and production via ERK/Ets-1 signaling. These results suggest that Lipo-PGE1 could potentially be a therapeutic target in diseases with deregulated collagen turnover. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Alprostadil / pharmacology* | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Collagen / antagonists & inhibitors* | - |
dc.subject.MESH | Collagen / metabolism | - |
dc.subject.MESH | Dermatologic Agents / pharmacology* | - |
dc.subject.MESH | Dermis / drug effects* | - |
dc.subject.MESH | Dermis / enzymology | - |
dc.subject.MESH | Fibroblasts / drug effects* | - |
dc.subject.MESH | Fibroblasts / enzymology | - |
dc.subject.MESH | Flavonoids / pharmacology | - |
dc.subject.MESH | Gene Expression / drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Keloid / drug therapy | - |
dc.subject.MESH | Keloid / enzymology | - |
dc.subject.MESH | MAP Kinase Signaling System / drug effects* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microspheres | - |
dc.subject.MESH | Protective Agents / pharmacology | - |
dc.subject.MESH | Protein Kinase Inhibitors / pharmacology | - |
dc.subject.MESH | Proto-Oncogene Protein c-ets-1 / metabolism | - |
dc.subject.MESH | RNA, Messenger / antagonists & inhibitors | - |
dc.subject.MESH | RNA, Messenger / metabolism | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.title | Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics and Gynecology (산부인과학교실) | - |
dc.contributor.googleauthor | Yoolhee Yang | - |
dc.contributor.googleauthor | Hee Jung Kim | - |
dc.contributor.googleauthor | Kyong-Je Woo | - |
dc.contributor.googleauthor | Daeho Cho | - |
dc.contributor.googleauthor | Sa Ik Bang | - |
dc.identifier.doi | 10.1371/journal.pone.0179614 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 28644845 | - |
dc.citation.volume | 12 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | e0179614 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.12(6) : e0179614, 2017-06 | - |
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