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Organization of the 5' region of the rat ATP-citrate lyase gene
DC Field | Value | Language |
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dc.contributor.author | 박상욱 | - |
dc.date.accessioned | 2023-07-12T00:37:36Z | - |
dc.date.available | 2023-07-12T00:37:36Z | - |
dc.date.issued | 1994-09 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195226 | - |
dc.description.abstract | A genomic clone, encompassing the 5' flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5' flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5' flanking region (-2370 to -1) in the CHO cell line. The 5' flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Portland Press on behalf of the Biochemical Society | - |
dc.relation.isPartOf | BIOCHEMICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | ATP Citrate (pro-S)-Lyase / genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Blotting, Southern | - |
dc.subject.MESH | Electrophoresis, Agar Gel | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nucleic Acid Hybridization | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Regulatory Sequences, Nucleic Acid | - |
dc.subject.MESH | Transcription, Genetic / genetics | - |
dc.subject.MESH | Transfection | - |
dc.title | Organization of the 5' region of the rat ATP-citrate lyase gene | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Kyung-Sup KIM | - |
dc.contributor.googleauthor | Sahng-Wook PARK | - |
dc.contributor.googleauthor | Young-Ah MOON | - |
dc.contributor.googleauthor | Yoon-Soo KIM | - |
dc.identifier.doi | 10.1042/bj3020759 | - |
dc.contributor.localId | A01487 | - |
dc.relation.journalcode | J00282 | - |
dc.identifier.eissn | 1470-8728 | - |
dc.identifier.pmid | 7945200 | - |
dc.contributor.alternativeName | Park, Sahng Wook | - |
dc.contributor.affiliatedAuthor | 박상욱 | - |
dc.citation.volume | 302 | - |
dc.citation.number | Pt 3 | - |
dc.citation.startPage | 759 | - |
dc.citation.endPage | 764 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL JOURNAL, Vol.302(Pt 3) : 759-764, 1994-09 | - |
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