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A new endothelial molecule involved in melanoma cell binding to human dermal microvascular endothelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이광훈 | - |
dc.contributor.author | 정기양 | - |
dc.date.accessioned | 2023-07-12T00:36:35Z | - |
dc.date.available | 2023-07-12T00:36:35Z | - |
dc.date.issued | 1994-01 | - |
dc.identifier.issn | 1013-9087 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195214 | - |
dc.description.abstract | Background: We have previously demonstrated that phorbol myristate acetate (PMA) -induced increases in melanoma cell binding to endothelial cells derived from human dermis (HDMEC) are not mediated via known cell adhesion molecules and may be affected through microvessel-specific novel proteins not previously described on endothelial cells. Objective: This study was performed to identify new molecules which may play a role in HDMEC-melanoma cells binding. Methods: We have generated a monoclonal antibody(Mab) against PMA-stimulated HDMEC. A Mab was evaluated functionally through melanoma cell-endothelial cell adherence assay and characterized by Western immunoblot. Results: Mab EM-71 recognized a molecule with expression levels in vitro that could be upregulated by PMA(EM-71 molecule). The expression of EM-71 molecule on HDMEC was increased in a dose-dependent manner by PMA only, but not affected by interleukin 1 alpha(IL-la) or tumor necrosis factor alpha(TNFa) PMA augmented melanoma cell adherence to HDMEC, which is coincident with an increase in EM-71 molecule expression on HDMEC by PMA. Mab EM-71 partially inhibited up to 59% of the increased melanoma cell binding to PMA-stimulated HDMEC and failed to block melanoma cell binding to IL-la or TNFa-stimulated HDMEC. Western immunoblots of lysates of HDMEC demonstrated a 200 kDa protein on HDMEC. Conclusion: This study demonstrates that EM-71 molecule may play a partial role in melanoma binding to PMA-stimulated HDMEC. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Korean Dermatological Association | - |
dc.relation.isPartOf | ANNALS OF DERMATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | A new endothelial molecule involved in melanoma cell binding to human dermal microvascular endothelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.contributor.googleauthor | Kee Yang Chung | - |
dc.contributor.googleauthor | Thomas J. Lawley | - |
dc.contributor.googleauthor | Robert A. Swerlick | - |
dc.contributor.googleauthor | Lee Kwang Hoon | - |
dc.contributor.googleauthor | Chung Kee-Yang | - |
dc.contributor.localId | A02674 | - |
dc.contributor.localId | A03582 | - |
dc.relation.journalcode | J00158 | - |
dc.identifier.eissn | 2005-3894 | - |
dc.identifier.url | https://kiss.kstudy.com/thesis/thesis-view.asp?key=2452734 | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.affiliatedAuthor | 이광훈 | - |
dc.contributor.affiliatedAuthor | 정기양 | - |
dc.citation.volume | 6 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 6 | - |
dc.citation.endPage | 16 | - |
dc.identifier.bibliographicCitation | ANNALS OF DERMATOLOGY, Vol.6(1) : 6-16, 1994-01 | - |
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