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Effect of hypothermic cardioplegia on cardiac protection -I. Effect of hypothermic cardioplegia on the cytosolic Ca2+ concentration in rat ventricular myocytes-
DC Field | Value | Language |
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dc.contributor.author | 안덕선 | - |
dc.contributor.author | 이영호 | - |
dc.date.accessioned | 2023-07-12T00:31:20Z | - |
dc.date.available | 2023-07-12T00:31:20Z | - |
dc.date.issued | 1994-06 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195156 | - |
dc.description.abstract | Cytosolic Ca2+ concentration of rat ventricular cells was measured under varying experimental conditions by using a fluorescent Ca2+ indicator, Fura-2. Resting [Ca2+]i of rat myocyte was 150 ± 30 nM (n = 39), and this value was compatible with others. The Perfusion of cardioplegic solution significantly increased [Ca2+]i, and this effect was further augmented by hypothermia (p<0.05). Application of nifedipine (5 x 10-7 M) to the perfusate or pretreatment of caffeine (10 mM) had no apparent effect on this cardioplegia-induced [Ca2+]i change. But Ni2+ (5 mM), an antagonist of Na+/Ca2+ exchange mechanism, prevented the [Ca2+]i change during cardioplegia (p<0.05). Magnitude of cardioplegia-induced [Ca2+]i increase was also dependent on the Ca2+ concentration of cardioplegic solution. These results suggest that Na+/Ca2+ exchange may play an important role in cardioplegia-induced [Ca2+]i change. To rule out the possibility whether the protective effect of hypothermic cardioplegia is due to the preservation of high-energy phosphate store or decreasing the transmembrane ionic fluxes by phase transition, we exhausted a energy store of cardiac cell by application of 2,4 dinitrophenol to the bath and measured its effect on [Ca2+]i change during cardioplegia. Hypothermic cardioplegia delayed the onset of [Ca2+]i increase and decreased its amplitude compared to those of normothermic cardioplegia. From the above results, hypothermic cardioplegia may protect the cardiac cells from ischemic insult by preserving a high-energy phosphate store. Application of Ni2+ to the cardioplegic solution or reduction of external Ca2+ concentration also had some protective effect, since it prevented [Ca2+]i increase during cardioplegia. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium / metabolism* | - |
dc.subject.MESH | Cytosol / metabolism | - |
dc.subject.MESH | Heart Arrest, Induced* | - |
dc.subject.MESH | Heart Ventricles / metabolism | - |
dc.subject.MESH | Hypothermia, Induced | - |
dc.subject.MESH | Myocardial Ischemia / metabolism | - |
dc.subject.MESH | Myocardial Ischemia / prevention & control* | - |
dc.subject.MESH | Myocardium / metabolism* | - |
dc.subject.MESH | Rats | - |
dc.title | Effect of hypothermic cardioplegia on cardiac protection -I. Effect of hypothermic cardioplegia on the cytosolic Ca2+ concentration in rat ventricular myocytes- | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학교실) | - |
dc.contributor.googleauthor | Duck Sun Ahn | - |
dc.contributor.googleauthor | Young Ho Lee | - |
dc.contributor.googleauthor | Doo Hee Kang | - |
dc.contributor.googleauthor | Bok Soon Kang | - |
dc.identifier.doi | 10.3349/ymj.1994.35.2.162 | - |
dc.contributor.localId | A02223 | - |
dc.contributor.localId | A02968 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 8091793 | - |
dc.contributor.alternativeName | Ahn, Duk Sun | - |
dc.contributor.affiliatedAuthor | 안덕선 | - |
dc.contributor.affiliatedAuthor | 이영호 | - |
dc.citation.volume | 35 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 162 | - |
dc.citation.endPage | 176 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.35(2) : 162-176, 1994-06 | - |
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