Kawasaki환자에서 HSP65에 대한 항체의 검출

Abstract

Kawasaki disease is an acute febrile illness affecting maily infants and children and characterized by systemic vasculitis with multiple immunologic abnormalities, Heat shock proteins have many functions such as inflammation and autoimmunity. To observe the role of heat shock protein in the Kawasaki disease, antibody responses to mycobacterial 65kDa protein were examined by ELISA and immunoblotting using acute and subacute phase sera of 10 patients with Kawasaki disease. Markedly increased antibody titer against antigen of BCG and perchloric acid extracted mycobacterial 65kDa protein were observed in the subacute sera compared to their acute sera, but not against purified protein derivatives(PPD) and lipoarabinomannan(LAM). By immunoblotting, the subacute sera reacted with 65 kDa protein strongly and other proteins of sonicated BCG antigen, and also reacted with 65 kDa protein of perchloric acid soluble BCG antigen, but the acute serum did not. These results indicated that BCG extracted mycobacterial heat shock protein 65 might have play some important roles in pathogenesis of Kawasaki disease 2 we suggest autoreactivity to host HSP65 similar to mucobacterial HSP65 and further study on the roles or functions of this mycobacterial heat shock protein 65 or the auto-reactivity to self HSP65 should be done.