자궁경부암 조직에서 유식세포분석기를 이용한 DNA 분석과 예후인자로서의 유용성

Abstract

The biological behaviour of malignant tumors were generally assessed by morphological cell grading, clinical staging, and measuring various tumor markers. Recently DNA ploidy were measured by flow cytometry and suggested ans an additional useful indicator of the tumor behaviour. The DNA ploidy using paraffin-embedded tissues from 18 cervical cancer, 14 cervical intraepithelial neoplasia and 11 controls were analyzed by flow cytometry in order to evaluate the correlation between DNA ploidy patterns and prognostic factors of cervical cancer. DNA aneuploidy, S-phase fraction and DNA index were statistically higher in cases of CIN and cervical cancer compared to controls. And no significant differences were detected in DNA aneuploidy, S-phases fraction, DNA index between the stage of disease or the other prognostic factors, but the aneuploidy level were higher in cases of deeper invasion depth, positive lymph node metastasia and adenocarcinoma. This results suggest that the DNA ploidy level can be used as an independent prognostic factor, but further evaluation will be needed in order to conclude definite relationship between prognostic factors and S-phase fraction or DNA index.