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Efficient gene transfection to lung cancer cells via Folate-PEI-Sorbitol gene transporter
DC Field | Value | Language |
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dc.contributor.author | 천재희 | - |
dc.date.accessioned | 2023-06-02T00:52:02Z | - |
dc.date.available | 2023-06-02T00:52:02Z | - |
dc.date.issued | 2022-05 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/194483 | - |
dc.description.abstract | Lung cancer is known to be one of the fatal diseases in the world and is experiencing treatment difficulties. Many treatments have been discovered and implemented, but death rate of patients with lung cancer continues to remain high. Current treatments for cancer such as chemotherapy, immunotherapy, and radiotherapy have shown considerable results, yet they are accompanied by side effects. One effective method for reducing the cytotoxicity of these treatments is via the use of a nanoparticle-mediated siRNA delivery strategy with selective silencing effects and non-viral vectors. In this study, a folate (FA) moiety ligand-conjugated poly(sorbitol-co-PEI)-based gene transporter was designed by combining low-molecular weight polyethyleneimine (LMW PEI) and D-sorbitol with FA to form FPS. Since folate receptors are commonly overexpressed in various cancer cells, folate-conjugated nanoparticles may be more effectively delivered to selective cancer cells. Additionally, siOPA1 was used to induce apoptosis through mitochondrial fusion. The OPA1 protein stability level is important for maintaining normal mitochondrial cristae structure and function, conserving the inner membrane structure, and protecting cells from apoptosis. Consequently, when FPS/siOPA1 was used for lung cancer in-vitro and in-vivo, it improved cell viability and cellular uptake. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Folic Acid / metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lung Neoplasms* / genetics | - |
dc.subject.MESH | Lung Neoplasms* / therapy | - |
dc.subject.MESH | Polyethyleneimine / chemistry | - |
dc.subject.MESH | RNA, Small Interfering / metabolism | - |
dc.subject.MESH | Sorbitol* | - |
dc.subject.MESH | Transfection | - |
dc.title | Efficient gene transfection to lung cancer cells via Folate-PEI-Sorbitol gene transporter | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Kye Soo Cho | - |
dc.contributor.googleauthor | Sanghwa Kim | - |
dc.contributor.googleauthor | Hyung Bin Chun | - |
dc.contributor.googleauthor | Jae Hee Cheon | - |
dc.contributor.googleauthor | Myung-Haing Cho | - |
dc.contributor.googleauthor | Ah Young Lee | - |
dc.contributor.googleauthor | Rohidas B Arote | - |
dc.identifier.doi | 10.1371/journal.pone.0266181 | - |
dc.contributor.localId | A04030 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 35507584 | - |
dc.contributor.alternativeName | Cheon, Jae Hee | - |
dc.contributor.affiliatedAuthor | 천재희 | - |
dc.citation.volume | 17 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | e0266181 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.17(5) : e0266181, 2022-05 | - |
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