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Detection of nosocomial pneumonia pathogens using a fluorescence-based device
DC Field | Value | Language |
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dc.contributor.author | 양지혜 | - |
dc.date.accessioned | 2023-06-02T00:47:23Z | - |
dc.date.available | 2023-06-02T00:47:23Z | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 1572-1000 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/194434 | - |
dc.description.abstract | Background: Early detection of nosocomial pneumonia pathogens is a significant factor in hospital-acquired pneumonia care. This study aimed to determine the autofluorescence properties of five nosocomial pneumonia pathogens using a fluorescence-based device and to establish evidence for clinical guidelines. Methods: The following bacterial strains were assessed: Acinetobacter baumannii (AB), Escherichia coli (EC), Enterococcus faecalis (EF), Klebsiella pneumoniae (KP), and Staphylococcus aureus (SA). The bacteria were cultured separately on tryptic soy agar at 37 °C under aerobic conditions for 168 h. Fluorescence photographs of each species were captured every 24 h using a fluorescence-based device with fixed camera settings. The images were analyzed by measuring the red and green values (R/G ratio) at a central point in each colony, and the R/G ratios were analyzed using the Kruskal-Wallis non-parametric test. Results: KP and SA showed red fluorescence with their R/G values, which were significantly higher than those of the other strains (p < 0.001). In particular, the R/G ratio of KP increased steadily until 72 h of incubation, peaking at 3.65. In addition, AB and EC showed orange fluorescence with higher red ratios than green ratios. EF and SA showed green fluorescence all through 168 h of incubation, with R/G ratio less than 1.0. Conclusions: Nosocomial pneumonia pathogens can be identified and classified via bacterial autofluorescence emission. It is possible to develop a rapid and easy-to-use identification technology based on bacterial autofluorescence for clinical applications. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Anti-Bacterial Agents | - |
dc.subject.MESH | Cross Infection* | - |
dc.subject.MESH | Healthcare-Associated Pneumonia* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Photochemotherapy* / methods | - |
dc.title | Detection of nosocomial pneumonia pathogens using a fluorescence-based device | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Pathology (구강병리학교실) | - |
dc.contributor.googleauthor | Eun-Ha Jung | - |
dc.contributor.googleauthor | Ji-Hye Yang | - |
dc.contributor.googleauthor | So-Jung Mun | - |
dc.contributor.googleauthor | Sun-Young Han | - |
dc.identifier.doi | 10.1016/j.pdpdt.2021.102621 | - |
dc.contributor.localId | A05149 | - |
dc.relation.journalcode | J02519 | - |
dc.identifier.eissn | 1873-1597 | - |
dc.identifier.pmid | 34749000 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1572100021004397 | - |
dc.subject.keyword | Autofluorescence | - |
dc.subject.keyword | Fluorescence property | - |
dc.subject.keyword | Fluorescence-based device | - |
dc.subject.keyword | Nosocomial pneumonia pathogens | - |
dc.subject.keyword | Porphyrin | - |
dc.contributor.alternativeName | Yang, Ji Hye | - |
dc.contributor.affiliatedAuthor | 양지혜 | - |
dc.citation.volume | 37 | - |
dc.citation.startPage | 102621 | - |
dc.identifier.bibliographicCitation | PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, Vol.37 : 102621, 2022-03 | - |
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