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Exon definitive regions for MPC1 microexon splicing and its usage for splicing modulation

Authors
 Eunjin Koh  ;  Daye Shin  ;  Kyung-Sup Kim 
Citation
 MOLECULAR THERAPY-NUCLEIC ACIDS, Vol.31 : 398-410, 2023-01 
Journal Title
MOLECULAR THERAPY-NUCLEIC ACIDS
Issue Date
2023-01
Keywords
MT: RNA/DNA Editing ; alternative splicing ; exon definition ; intron definition ; microexon ; splicing
Abstract
Alternative splicing of microexons (3-30 base pairs [bp]) is involved in important biological processes in brain development and human cancers. However, understanding a splicing process of non-3x bp microexons is scarce. We showed that 4 bp microexon of mitochondrial pyruvate carrier1 (MPC1) is constitutively included in mRNA. Based on our studies with minigene and exon island constructs, we found the strong exon definition region in the proximal introns bordering MPC1 microexon. Ultimately, we defined a nucleotide fragment from the 3'ss 67 bp of MPC1 microexon to the 5'ss consensus sequence, as a core exon island, which can concatenate its microexon and neighboring exons by splicing. Furthermore, we showed that insertion of the core exon island into a target exon or intron induced skip the target exon or enhance the splicing of an adjacent exon, respectively. Collectively, we suggest that the exon island derived from MPC1 microexon modifies genuine splicing patterns depending on its position, thereby providing insights on strategies for splicing-mediated gene correction.
Files in This Item:
T202301594.pdf Download
DOI
10.1016/j.omtn.2023.01.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Koh, Eun Jin(고은진) ORCID logo https://orcid.org/0000-0001-8967-6266
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/193685
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