Cited 4 times in
Immobilized Amyloid Hexamer Fragments to Map Active Sites of Amyloid-Targeting Chemicals
DC Field | Value | Language |
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dc.contributor.author | 김어수 | - |
dc.date.accessioned | 2023-03-22T02:41:30Z | - |
dc.date.available | 2023-03-22T02:41:30Z | - |
dc.date.issued | 2023-01 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/193651 | - |
dc.description.abstract | As amyloid-β (Aβ) peptide is considered a biomarker and pathological culprit of Alzheimer's disease, Aβ-targeting compounds have been investigated for diagnostics development and drug discovery of the disorder. Unlike amyloid plaque targeting agents, such as clinically available amyloid radiotracers intercalating into the β-sheet structures of the aggregates, monomer and oligomer targeting chemicals are difficult to develop, as the transient and polymorphic nature of these peptides impedes their structural understanding. Here, we report a mapping approach to explore targeting residues of Aβ-imaging probes and Aβ-regulating drug candidates by utilizing a set of fragmented Aβ hexamers immobilized on a 96-well microplate in combination with fluorescent full-length Aβ for on-plate aggregation. To evaluate the mapping potential of the peptide plate, we tested previously reported fluorescent imaging agents (CRANAD-28, bis-ANS), aggregation inhibitors (curcumin, scyllo-inositol), and aggregate dissociators (necrostatin-1, sunitinib) targeting Aβ. Our approach enabled mechanistic understanding of compounds targeting nonfibrillar Aβ on an interacting sequence level. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS CHEMICAL NEUROSCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Alzheimer Disease* / pathology | - |
dc.subject.MESH | Amyloid | - |
dc.subject.MESH | Amyloid beta-Peptides* / chemistry | - |
dc.subject.MESH | Catalytic Domain | - |
dc.subject.MESH | Fluorescent Dyes | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Peptide Fragments / chemistry | - |
dc.title | Immobilized Amyloid Hexamer Fragments to Map Active Sites of Amyloid-Targeting Chemicals | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Psychiatry (정신과학교실) | - |
dc.contributor.googleauthor | Illhwan Cho | - |
dc.contributor.googleauthor | Soljee Yoon | - |
dc.contributor.googleauthor | Sunghyun Park | - |
dc.contributor.googleauthor | Seung Woo Hong | - |
dc.contributor.googleauthor | Eunjung Cho | - |
dc.contributor.googleauthor | Eosu Kim | - |
dc.contributor.googleauthor | Hye Yun Kim | - |
dc.contributor.googleauthor | YoungSoo Kim | - |
dc.identifier.doi | 10.1021/acschemneuro.2c00449 | - |
dc.contributor.localId | A00686 | - |
dc.relation.journalcode | J04257 | - |
dc.identifier.eissn | 1948-7193 | - |
dc.identifier.pmid | 36445044 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acschemneuro.2c00449 | - |
dc.subject.keyword | aggregate dissociators | - |
dc.subject.keyword | aggregation inhibitors | - |
dc.subject.keyword | amyloid-β | - |
dc.subject.keyword | fluorescent imaging agents | - |
dc.subject.keyword | mapping assay | - |
dc.subject.keyword | peptide synthesis | - |
dc.contributor.alternativeName | Kim, Eo Su | - |
dc.contributor.affiliatedAuthor | 김어수 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 9 | - |
dc.citation.endPage | 18 | - |
dc.identifier.bibliographicCitation | ACS CHEMICAL NEUROSCIENCE, Vol.14(1) : 9-18, 2023-01 | - |
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