Cited 3 times in
Single Cell Analysis Reveals Reciprocal Tumor-Macrophage Intercellular Communications Related with Metabolic Reprogramming in Stem-like Gastric Cancer
DC Field | Value | Language |
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dc.contributor.author | 정재호 | - |
dc.date.accessioned | 2023-03-10T01:32:13Z | - |
dc.date.available | 2023-03-10T01:32:13Z | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/193208 | - |
dc.description.abstract | Metabolic alterations and direct cell-cell interactions in the tumor microenvironment (TME) affect the prognostic molecular landscape of tumors; thus, it is imperative to investigate metabolic activity at the single-cell level rather than in bulk samples to understand the high-resolution mechanistic influences of cell-type specific metabolic pathway alterations on tumor cells. To investigate tumor metabolic reprogramming and intercellular communication at the single-cell level, we analyzed eighty-four metabolic pathways, seven metabolic signatures, and tumor-stroma cell interaction using 21,084 cells comprising gastric cancer and paired normal tissue. High EMT-score cells and stem-like subtype tumors showed elevated glycosaminoglycan metabolism, which was associated with poor patient outcome. Adenocarcinoma and macrophage cells had higher reactive oxidative species levels than the normal controls; they largely constituted the highest stemness cluster. They were found to reciprocally communicate through the common ligand RPS19. Consequently, ligand-target regulated transcriptional reprogramming resulted in HS6ST2 expression in adenocarcinoma cells and SERPINE1 expression in macrophages. Gastric cancer patients with increased SERPINE1 and HS6ST2 expression had unfavorable prognoses, suggesting these as potential drug targets. Our findings indicate that malignant stem-like/EMT cancer cell state might be regulated through reciprocal cancer cell-macrophage intercellular communication and metabolic reprogramming in the heterogeneous TME of gastric cancer at the single-cell level. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | CELLS(Cells) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adenocarcinoma* | - |
dc.subject.MESH | Cell Communication | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Ligands | - |
dc.subject.MESH | Macrophages / metabolism | - |
dc.subject.MESH | Single-Cell Analysis | - |
dc.subject.MESH | Stomach Neoplasms* / metabolism | - |
dc.subject.MESH | Sulfotransferases | - |
dc.subject.MESH | Tumor Microenvironment | - |
dc.title | Single Cell Analysis Reveals Reciprocal Tumor-Macrophage Intercellular Communications Related with Metabolic Reprogramming in Stem-like Gastric Cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Ji-Yong Sung | - |
dc.contributor.googleauthor | Jae-Ho Cheong | - |
dc.identifier.doi | 10.3390/cells11152373 | - |
dc.contributor.localId | A03717 | - |
dc.relation.journalcode | J03774 | - |
dc.identifier.pmid | 35954218 | - |
dc.subject.keyword | cancer stemness | - |
dc.subject.keyword | gastric cancer | - |
dc.subject.keyword | glycan metabolism | - |
dc.subject.keyword | immunometabolism | - |
dc.subject.keyword | single cell | - |
dc.contributor.alternativeName | Cheong, Jae Ho | - |
dc.contributor.affiliatedAuthor | 정재호 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 2373 | - |
dc.identifier.bibliographicCitation | CELLS, Vol.11(15) : 2373, 2022-08 | - |
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