Cited 5 times in
Gut Epithelial Inositol Polyphosphate Multikinase Alleviates Experimental Colitis via Governing Tuft Cell Homeostasis
DC Field | Value | Language |
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dc.contributor.author | 황성순 | - |
dc.date.accessioned | 2022-12-22T03:37:30Z | - |
dc.date.available | 2022-12-22T03:37:30Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191922 | - |
dc.description.abstract | Background & aims: Inositol polyphosphate multikinase (IPMK), an essential enzyme for inositol phosphate metabolism, has been known to mediate major biological events such as growth. Recent studies have identified single-nucleotide polymorphisms in the IPMK gene associated with inflammatory bowel disease predisposition. Therefore, we aimed to investigate the functional significance of IPMK in gut epithelium. Methods: We generated intestinal epithelial cell (IEC)-specific Ipmk knockout (IPMKΔIEC) mice, and assessed their vulnerability against dextran sulfate sodium-induced experimental colitis. Both bulk and single-cell RNA sequencing were performed to analyze IPMK-deficient colonic epithelial cells and colonic tuft cells. Results: Although IPMKΔIEC mice developed normally and showed no intestinal abnormalities during homeostasis, Ipmk deletion aggravated dextran sulfate sodium-induced colitis, with higher clinical colitis scores, and increased epithelial barrier permeability. Surprisingly, Ipmk deletion led to a significant decrease in the number of tuft cells without influencing other IECs. Single-cell RNA sequencing of mouse colonic tuft cells showed 3 distinct populations of tuft cells, and further showed that a transcriptionally inactive population was expanded markedly in IPMKΔIEC mice, while neuronal-related cells were relatively decreased. Conclusions: Cholinergic output from tuft cells is known to be critical for the restoration of intestinal architecture upon damage, supporting that tuft cell-defective IPMKΔIEC mice are more prone to colitis. Thus, intestinal epithelial IPMK is a critical regulator of colonic integrity and tissue regeneration by determining tuft cell homeostasis and affecting cholinergic output. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | American Gastroenterological Association | - |
dc.relation.isPartOf | CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Colitis* / chemically induced | - |
dc.subject.MESH | Colitis* / drug therapy | - |
dc.subject.MESH | Dextran Sulfate | - |
dc.subject.MESH | Homeostasis | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Phosphotransferases (Alcohol Group Acceptor) / genetics | - |
dc.title | Gut Epithelial Inositol Polyphosphate Multikinase Alleviates Experimental Colitis via Governing Tuft Cell Homeostasis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Seung Eun Park | - |
dc.contributor.googleauthor | Dongeun Lee | - |
dc.contributor.googleauthor | Jae Woong Jeong | - |
dc.contributor.googleauthor | Su-Hyung Lee | - |
dc.contributor.googleauthor | Seung Ju Park | - |
dc.contributor.googleauthor | Jaeseung Ryu | - |
dc.contributor.googleauthor | Se Kyu Oh | - |
dc.contributor.googleauthor | Hanseul Yang | - |
dc.contributor.googleauthor | Sungsoon Fang | - |
dc.contributor.googleauthor | Seyun Kim | - |
dc.identifier.doi | 10.1016/j.jcmgh.2022.08.004 | - |
dc.contributor.localId | A05443 | - |
dc.relation.journalcode | J03804 | - |
dc.identifier.eissn | 2352-345X | - |
dc.identifier.pmid | 35988719 | - |
dc.subject.keyword | Colitis | - |
dc.subject.keyword | Colon | - |
dc.subject.keyword | Epithelial Barrier | - |
dc.subject.keyword | IBD | - |
dc.subject.keyword | IPMK | - |
dc.subject.keyword | Single-Cell RNA-Seq | - |
dc.subject.keyword | Tuft Cell | - |
dc.contributor.alternativeName | Fang, Sungsoon | - |
dc.contributor.affiliatedAuthor | 황성순 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1235 | - |
dc.citation.endPage | 1256 | - |
dc.identifier.bibliographicCitation | CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, Vol.14(6) : 1235-1256, 2022-09 | - |
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