Cited 4 times in
Mass Cytometry Exploration of Immunomodulatory Responses of Human Immune Cells Exposed to Silver Nanoparticles
DC Field | Value | Language |
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dc.contributor.author | 송재우 | - |
dc.date.accessioned | 2022-12-22T01:41:43Z | - |
dc.date.available | 2022-12-22T01:41:43Z | - |
dc.date.issued | 2022-03 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191304 | - |
dc.description.abstract | Increasing production and application of silver nanoparticles (Ag NPs) have raised concerns on their possible adverse effects on human health. However, a comprehensive understanding of their effects on biological systems, especially immunomodulatory responses involving various immune cell types and biomolecules (e.g., cytokines and chemokines), is still incomplete. In this study, a single-cell-based, high-dimensional mass cytometry approach is used to investigate the immunomodulatory responses of Ag NPs using human peripheral blood mononuclear cells (hPBMCs) exposed to poly-vinyl-pyrrolidone (PVP)-coated Ag NPs of different core sizes (i.e., 10-, 20-, and 40-nm). Although there were no severe cytotoxic effects observed, PVPAg10 and PVPAg20 were excessively found in monocytes and dendritic cells, while PVPAg40 displayed more affinity with B cells and natural killer cells, thereby triggering the release of proinflammatory cytokines such as IL-2, IL-17A, IL-17F, MIP1β, TNFα, and IFNγ. Our findings indicate that under the exposure conditions tested in this study, Ag NPs only triggered the inflammatory responses in a size-dependent manner rather than induce cytotoxicity in hPBMCs. Our study provides an appropriate ex vivo model to better understand the human immune responses against Ag NP at a single-cell level, which can contribute to the development of targeted drug delivery, vaccine developments, and cancer radiotherapy treatments. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | PHARMACEUTICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Mass Cytometry Exploration of Immunomodulatory Responses of Human Immune Cells Exposed to Silver Nanoparticles | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jiwon Bae | - |
dc.contributor.googleauthor | My Ha | - |
dc.contributor.googleauthor | Haribalan Perumalsamy | - |
dc.contributor.googleauthor | Yangsoon Lee | - |
dc.contributor.googleauthor | Jaewoo Song | - |
dc.contributor.googleauthor | Tae-Hyun Yoon | - |
dc.identifier.doi | 10.3390/pharmaceutics14030630 | - |
dc.contributor.localId | A02054 | - |
dc.relation.journalcode | J02504 | - |
dc.identifier.eissn | 1999-4923 | - |
dc.identifier.pmid | 35336005 | - |
dc.subject.keyword | immune systems | - |
dc.subject.keyword | immunomodulatory responses | - |
dc.subject.keyword | mass cytometry | - |
dc.subject.keyword | silver nanoparticles | - |
dc.subject.keyword | single-cell analysis | - |
dc.contributor.alternativeName | Song, Jae Woo | - |
dc.contributor.affiliatedAuthor | 송재우 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 630 | - |
dc.identifier.bibliographicCitation | PHARMACEUTICS, Vol.14(3) : 630, 2022-03 | - |
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