Cited 6 times in
Trajectory of genetic alterations associated with colistin resistance in Acinetobacter baumannii during an in-hospital outbreak of infection
DC Field | Value | Language |
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dc.contributor.author | 원동주 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 최종락 | - |
dc.contributor.author | 윤은정 | - |
dc.contributor.author | 윤은정 | - |
dc.date.accessioned | 2022-12-22T01:23:37Z | - |
dc.date.available | 2022-12-22T01:23:37Z | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0305-7453 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191192 | - |
dc.description.abstract | Background: As carbapenem-resistant Acinetobacter baumannii is dominant in clinical settings, the old polymyxin antibiotic colistin has been revived as a therapeutic option. The development of colistin resistance during treatment is becoming a growing concern. Objectives: To access low- to mid-level colistin-resistant A. baumannii blood isolates recovered from an outbreak in a tertiary care hospital from a national antimicrobial surveillance study. Methods: The entire bacterial genome was sequenced through long-read sequencing methodology. Quantitative RT-PCR was carried out to determine the level of gene expression. Relative growth rates were determined to estimate fitness costs of each isolate caused by the genetic alterations. Results: The A. baumannii isolates belonged to global clone 2 harbouring two intrinsic phosphoethanolamine transferases. Cumulative alterations continuing the colistin resistance were observed. PmrC overproduction caused by the PmrBA226T alteration was identified in A. baumannii isolates with low-level colistin resistance and an additional PmrCR109H substitution led to mid-level colistin resistance. Truncation of the PmrC enzyme by insertion of ISAba59 was compensated by ISAba10-mediated overproduction of EptA and, in the last isolate, the complete PmrAB two-component regulatory system was eliminated to restore the biological cost of the bacterial host. Conclusions: During the in-hospital outbreak, a trajectory of genetic modification in colistin-resistant A. baumannii isolates was observed for survival in the harsh conditions imposed by life-threatening drugs with the clear purpose of maintaining drug resistance above a certain level with a reasonable fitness cost. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Oxford University Press | - |
dc.relation.isPartOf | JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Acinetobacter Infections* / microbiology | - |
dc.subject.MESH | Acinetobacter baumannii* | - |
dc.subject.MESH | Anti-Bacterial Agents / therapeutic use | - |
dc.subject.MESH | Bacterial Proteins / genetics | - |
dc.subject.MESH | Bacterial Proteins / metabolism | - |
dc.subject.MESH | Colistin / pharmacology | - |
dc.subject.MESH | Colistin / therapeutic use | - |
dc.subject.MESH | Disease Outbreaks | - |
dc.subject.MESH | Drug Resistance, Bacterial / genetics | - |
dc.subject.MESH | Drug Resistance, Multiple, Bacterial / genetics | - |
dc.subject.MESH | Hospitals | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.title | Trajectory of genetic alterations associated with colistin resistance in Acinetobacter baumannii during an in-hospital outbreak of infection | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Eun-Jeong Yoon | - |
dc.contributor.googleauthor | Hyun Soo Kim | - |
dc.contributor.googleauthor | Heungjeong Woo | - |
dc.contributor.googleauthor | You Jeong Choi | - |
dc.contributor.googleauthor | Dongju Won | - |
dc.contributor.googleauthor | Jong Rak Choi | - |
dc.contributor.googleauthor | Young Ah Kim | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.identifier.doi | 10.1093/jac/dkab363 | - |
dc.contributor.localId | A05763 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A04182 | - |
dc.relation.journalcode | J01237 | - |
dc.identifier.eissn | 1460-2091 | - |
dc.identifier.pmid | 34609499 | - |
dc.identifier.url | https://academic.oup.com/jac/article/77/1/69/6381549?login=true | - |
dc.contributor.alternativeName | Won, Dongju | - |
dc.contributor.affiliatedAuthor | 원동주 | - |
dc.contributor.affiliatedAuthor | 정석훈 | - |
dc.contributor.affiliatedAuthor | 최종락 | - |
dc.citation.volume | 77 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 69 | - |
dc.citation.endPage | 73 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol.77(1) : 69-73, 2022-01 | - |
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