Cited 8 times in
The HBV Core Protein and Core Particle Both Bind to the PPiase Par14 and Par17 to Enhance Their Stabilities and HBV Replication
DC Field | Value | Language |
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dc.contributor.author | 이현웅 | - |
dc.contributor.author | 임진홍 | - |
dc.date.accessioned | 2022-11-24T00:59:12Z | - |
dc.date.available | 2022-11-24T00:59:12Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191112 | - |
dc.description.abstract | We recently reported that the PPIase Par14 and Par17 encoded by PIN4 upregulate HBV replication in an HBx-dependent manner by binding to conserved arginine-proline (RP) motifs of HBx. HBV core protein (HBc) has a conserved 133RP134 motif; therefore, we investigated whether Par14/Par17 bind to HBc and/or core particles. Native agarose gel electrophoresis (NAGE) and immunoblotting and co-immunoprecipitation were used. Chromatin immunoprecipitation from HBV-infected HepG2-hNTCP-C9 cells was performed. NAGE and immunoblotting revealed that Par14/Par17 bound to core particles and co-immunoprecipitation revealed that Par14/Par17 interacted with core particle assembly-defective, and dimer-positive HBc-Y132A. Thus, core particles and HBc interact with Par14/Par17. Par14/Par17 interacted with the HBc 133RP134 motif possibly via substrate-binding E46/D74 and E71/D99 motifs. Although Par14/Par17 dissociated from core particles upon heat treatment, they were detected in 0.2 N NaOH-treated opened-up core particles, demonstrating that Par14/Par17 bind outside and inside core particles. Furthermore, these interactions enhanced the stabilities of HBc and core particles. Like HBc-Y132A, HBc-R133D and HBc-R133E were core particle assembly-defective and dimer-positive, demonstrating that a negatively charged residue at position 133 cannot be tolerated for particle assembly. Although positively charged R133 is solely important for Par14/17 interactions, the 133RP134 motif is important for efficient HBV replication. Chromatin immunoprecipitation from HBV-infected cells revealed that the S19 and E46/D74 residues of Par14 and S44 and E71/D99 residues of Par17 were involved in recruitment of 133RP134 motif-containing HBc into cccDNA. Our results demonstrate that interactions of HBc, Par14/Par17, and cccDNA in the nucleus and core particle-Par14/Par17 interactions in the cytoplasm are important for HBV replication. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Frontiers Research Foundation | - |
dc.relation.isPartOf | FRONTIERS IN MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | The HBV Core Protein and Core Particle Both Bind to the PPiase Par14 and Par17 to Enhance Their Stabilities and HBV Replication | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Umar Saeed | - |
dc.contributor.googleauthor | Zahra Zahid Piracha | - |
dc.contributor.googleauthor | Hyeonjoong Kwon | - |
dc.contributor.googleauthor | Jumi Kim | - |
dc.contributor.googleauthor | Fadia Kalsoom | - |
dc.contributor.googleauthor | Yong-Joon Chwae | - |
dc.contributor.googleauthor | Sun Park | - |
dc.contributor.googleauthor | Ho-Joon Shin | - |
dc.contributor.googleauthor | Hyun Woong Lee | - |
dc.contributor.googleauthor | Jin Hong Lim | - |
dc.contributor.googleauthor | Kyongmin Kim | - |
dc.identifier.doi | 10.3389/fmicb.2021.795047 | - |
dc.contributor.localId | A03292 | - |
dc.contributor.localId | A03411 | - |
dc.relation.journalcode | J03413 | - |
dc.identifier.eissn | 1664-302X | - |
dc.identifier.pmid | 34970249 | - |
dc.subject.keyword | HBV replication study | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | PPIase activity | - |
dc.subject.keyword | parvulin 14 | - |
dc.subject.keyword | parvulin 17 | - |
dc.contributor.alternativeName | Lee, Hyun Woong | - |
dc.contributor.affiliatedAuthor | 이현웅 | - |
dc.contributor.affiliatedAuthor | 임진홍 | - |
dc.citation.volume | 12 | - |
dc.citation.startPage | 795047 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN MICROBIOLOGY, Vol.12 : 795047, 2021-12 | - |
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