Cited 7 times in
Novel indel mutation in the N gene of SARS-CoV-2 clinical samples that were diagnosed positive in a commercial RT-PCR assay
DC Field | Value | Language |
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dc.contributor.author | 원동주 | - |
dc.contributor.author | 최종락 | - |
dc.date.accessioned | 2022-11-24T00:45:30Z | - |
dc.date.available | 2022-11-24T00:45:30Z | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 0168-1702 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190941 | - |
dc.description.abstract | Commercially available reverse transcription-polymerase chain reaction (RT-PCR) kits are being used as an important tool to diagnose SARS-CoV-2 infection in clinical laboratories worldwide. However, some kits lack sufficient clinical evaluation due to the need for emergency use caused by the current COVID-19 pandemic. Here we found that a novel insertion/deletion mutation in the nucleocapsid (N) gene of SARS-CoV-2 samples is a cause of negative results for the N gene in a widely used assay that received emergency use authorization (EUA) from US FDA and Conformite Europeenne-in vitro diagnostics (CE-IVD) from EU. Although SARS-CoV-2 is diagnosed positive by other target probes in the assay, our findings provide an evidence of the genetic variability and rapid evolution of SARS-CoV-2 as well as a reference in designing commercial RT-PCR assays. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Elsevier Science | - |
dc.relation.isPartOf | VIRUS RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | COVID-19 / diagnosis | - |
dc.subject.MESH | COVID-19 / virology* | - |
dc.subject.MESH | COVID-19 Nucleic Acid Testing | - |
dc.subject.MESH | Coronavirus Nucleocapsid Proteins / genetics* | - |
dc.subject.MESH | False Negative Reactions | - |
dc.subject.MESH | Genes, Viral | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | INDEL Mutation* | - |
dc.subject.MESH | Mass Screening | - |
dc.subject.MESH | Pandemics | - |
dc.subject.MESH | RNA, Viral / analysis | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | SARS-CoV-2 / genetics* | - |
dc.subject.MESH | SARS-CoV-2 / isolation & purification | - |
dc.title | Novel indel mutation in the N gene of SARS-CoV-2 clinical samples that were diagnosed positive in a commercial RT-PCR assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Sanghoo Lee | - |
dc.contributor.googleauthor | DongJu Won | - |
dc.contributor.googleauthor | Chang-Ki Kim | - |
dc.contributor.googleauthor | Jinwoo Ahn | - |
dc.contributor.googleauthor | Youngkee Lee | - |
dc.contributor.googleauthor | Hyeongkyun Na | - |
dc.contributor.googleauthor | Yun-Tae Kim | - |
dc.contributor.googleauthor | Mi-Kyeong Lee | - |
dc.contributor.googleauthor | Jong Rak Choi | - |
dc.contributor.googleauthor | Hwan-Sub Lim | - |
dc.contributor.googleauthor | Kyoung-Ryul Lee | - |
dc.identifier.doi | 10.1016/j.virusres.2021.198398 | - |
dc.contributor.localId | A05763 | - |
dc.contributor.localId | A04182 | - |
dc.relation.journalcode | J02786 | - |
dc.identifier.eissn | 1872-7492 | - |
dc.identifier.pmid | 33753180 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168170221001052 | - |
dc.subject.keyword | Different geographical origins | - |
dc.subject.keyword | Indel mutation | - |
dc.subject.keyword | Nucleocapsid gene | - |
dc.subject.keyword | SARS-CoV-2 | - |
dc.subject.keyword | Viral genome sequencing | - |
dc.contributor.alternativeName | Won, Dongju | - |
dc.contributor.affiliatedAuthor | 원동주 | - |
dc.contributor.affiliatedAuthor | 최종락 | - |
dc.citation.volume | 297 | - |
dc.citation.startPage | 198398 | - |
dc.identifier.bibliographicCitation | VIRUS RESEARCH, Vol.297 : 198398, 2021-05 | - |
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