0 28

Cited 0 times in

Genetic and epigenetic changes in the eutopic endometrium of women with endometriosis: association with decreased endometrial αvβ3 integrin expression

Authors
 Niraj R Joshi  ;  Hamid-Reza Kohan-Ghadr  ;  Damian S Roqueiro  ;  Jung Yoon Yoo  ;  Karenne Fru  ;  Eli Hestermann  ;  Lingwen Yuan  ;  Shuk-Mei Ho  ;  Jae-Wook Jeong  ;  Steven L Young  ;  Bruce A Lessey  ;  Asgerally T Fazleabas 
Citation
 MOLECULAR HUMAN REPRODUCTION, Vol.27(6), 2021-05 
Journal Title
MOLECULAR HUMAN REPRODUCTION
ISSN
 1360-9947 
Issue Date
2021-05
MeSH
Adolescent ; Adult ; Basic Helix-Loop-Helix Transcription Factors / biosynthesis ; Basic Helix-Loop-Helix Transcription Factors / genetics ; Biopsy ; DNA Methylation* ; Down-Regulation ; Endometriosis / complications ; Endometriosis / genetics* ; Endometriosis / metabolism ; Endometrium / metabolism* ; Endometrium / pathology ; Female ; Humans ; Infertility, Female / etiology* ; Infertility, Female / genetics ; Integrin alphaVbeta3 / biosynthesis* ; Integrin alphaVbeta3 / genetics ; Middle Aged ; Principal Component Analysis ; Receptors, Aryl Hydrocarbon / biosynthesis ; Receptors, Aryl Hydrocarbon / genetics ; Transcriptome* ; Young Adult
Keywords
aryl hydrocarbon receptor ; endometriosis ; epigenetics ; eutopic endometrium ; gene expression ; infertility ; integrin αvβ3 ; methylation
Abstract
About 40% of women with infertility and 70% of women with pelvic pain suffer from endometriosis. The pregnancy rate in women undergoing IVF with low endometrial integrin αvβ3 (LEI) expression is significantly lower compared to the women with high endometrial integrin αvβ3 (HEI). Mid-secretory eutopic endometrial biopsies were obtained from healthy controls (C; n=3), and women with HEI (n=4) and LEI (n=4) and endometriosis. Changes in gene expression were assessed using human gene arrays and DNA methylation data were derived using 385 K Two-Array Promoter Arrays. Transcriptional analysis revealed that LEI and C groups clustered separately with 396 differentially expressed genes (DEGs) (P<0.01: 275 up and 121 down) demonstrating that transcriptional and epigenetic changes are distinct in the LEI eutopic endometrium compared to the C and HEI group. In contrast, HEI vs C and HEI vs LEI comparisons only identified 83 and 45 DEGs, respectively. The methylation promoter array identified 1304 differentially methylated regions in the LEI vs C comparison. The overlap of gene and methylation array data identified 14 epigenetically dysregulated genes and quantitative RT-PCR analysis validated the transcriptomic findings. The analysis also revealed that aryl hydrocarbon receptor (AHR) was hypomethylated and significantly overexpressed in LEI samples compared to C. Further analysis validated that AHR transcript and protein expression are significantly (P<0.05) increased in LEI women compared to C. The increase in AHR, together with the altered methylation status of the 14 additional genes, may provide a diagnostic tool to identify the subset of women who have endometriosis-associated infertility.
DOI
10.1093/molehr/gaab018
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoo, Jung Yoon(유정윤) ORCID logo https://orcid.org/0000-0001-9366-3863
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190902
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links