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Neutralization of hexokinase 2-targeting miRNA attenuates the oxidative stress-induced cardiomyocyte apoptosis
DC Field | Value | Language |
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dc.contributor.author | 유경종 | - |
dc.date.accessioned | 2022-11-24T00:41:02Z | - |
dc.date.available | 2022-11-24T00:41:02Z | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 1386-0291 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190893 | - |
dc.description.abstract | Hexokinase 2 (HK2) is a metabolic sensor that couples glycolysis and oxidative phosphorylation of mitochondria by binding to the outer mitochondrial membrane (OMM), and it also has been implicated in induction of apoptotic process by regulating the integrity of OMM. When HK2 detaches from the mitochondria, it triggers permeability increase of the OMM and subsequently facilitates the cytosolic release of cytochrome c, a major apoptosis-inducing factor. According to previous studies, a harsh microenvironment created by ischemic heart disease such as low tissue oxygen and nutrients, and increased reactive oxygen species (ROS) can cause cardiomyocyte apoptosis. Under these conditions, the expression of HK2 in heart significantly decrease and such down-regulation of HK2 was correlated to the increased apoptosis of cardiomyocytes. Therefore, prevention of HK2 down-regulation may salvage cardiomyocytes from apoptosis. MicroRNAs are short, non-coding RNAs that either inhibit transcription of target mRNAs or degrade the targeted mRNAs via complementary binding to the 3'UTR (untranslated region) of the targeted mRNAs. Since miRNAs are known to be involved in virtually every biological processes, it is reasonable to assume that the expression of HK2 is also regulated by miRNAs. Currently, to my best knowledge, there is no previous study examined the miRNA-mediated regulation of HK2 in cardiomyocytes. Thus, in the present study, miRNA-mediated modulation of HK2 during ROS (H2O2)-induced cardiomyocyte apoptosis was investigated. First, the expression of HK2 in cardiomyocytes exposed to H2O2 was evaluated. H2O2 (500 μM) induced cardiomyocyte apoptosis and it also decreased the mitochondrial expression of HK2. Based on miRNA-target prediction databases and empirical data, miR-181a was identified as a HK2-targeting miRNA. To further examine the effect of negative regulation of the selected HK2-targeting miRNA on cardiomyocyte apoptosis, anti-miR-181a, which neutralizes endogenous miR-181a, was utilized. Delivery of anti-miR-181a significantly abrogated the H2O2-induced suppression of HK2 expression and subsequent disruption of mitochondrial membrane potential, improving the survival of cardiomyocytes exposed to H2O2. These findings suggest that miR-181a-mediated down-regulation of HK2 contributes to the apoptosis of cardiomyocytes exposed to ROS. Neutralizing miR-181a can be a viable and effective means to prevent cardiomyocyte from apoptosis in ischemic heart disease. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Ios Press | - |
dc.relation.isPartOf | CLINICAL HEMORHEOLOGY AND MICROCIRCULATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Hexokinase / metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | MicroRNAs / metabolism* | - |
dc.subject.MESH | Myocytes, Cardiac / metabolism* | - |
dc.subject.MESH | Oxidative Stress | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species | - |
dc.subject.MESH | Transfection | - |
dc.title | Neutralization of hexokinase 2-targeting miRNA attenuates the oxidative stress-induced cardiomyocyte apoptosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) | - |
dc.contributor.googleauthor | Kwan Wook Kim | - |
dc.contributor.googleauthor | Sang Woo Kim | - |
dc.contributor.googleauthor | Soyeon Lim | - |
dc.contributor.googleauthor | Kyung-Jong Yoo | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.contributor.googleauthor | Seahyoung Lee | - |
dc.identifier.doi | 10.3233/CH-200924 | - |
dc.contributor.localId | A02453 | - |
dc.relation.journalcode | J00576 | - |
dc.identifier.eissn | 1875-8622 | - |
dc.identifier.pmid | 33523042 | - |
dc.identifier.url | https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch200924 | - |
dc.subject.keyword | Hexokinase 2 | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | cardiomyocytes | - |
dc.subject.keyword | ischemic heart disease | - |
dc.subject.keyword | miR-181a | - |
dc.subject.keyword | reactive oxygen species | - |
dc.contributor.alternativeName | Yoo, Kyung Jong | - |
dc.contributor.affiliatedAuthor | 유경종 | - |
dc.citation.volume | 78 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 57 | - |
dc.citation.endPage | 68 | - |
dc.identifier.bibliographicCitation | CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, Vol.78(1) : 57-68, 2021-06 | - |
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