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Lithium chloride inhibits the migration and invasion of osteosarcoma cells by blocking nuclear translocation of phospho-Erk

DC Field Value Language
dc.contributor.author김주영-
dc.contributor.author용태순-
dc.date.accessioned2022-09-14T01:49:09Z-
dc.date.available2022-09-14T01:49:09Z-
dc.date.issued2021-12-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/190626-
dc.description.abstractLithium chloride (LiCl) is an important mood-stabilizing therapeutic agent for bipolar disorders, which has also been shown to inhibit cancer cell metastasis. Investigations of LiCl-induced signaling have focused mainly on extracellular signal regulated kinase 1/2 (ERK1/2) and glycogen synthase kinase 3 (GSK-3). However, little is known about the differences in cellular activities resulting from specific signaling via each of these pathways. In this study, we investigated the difference in responses between the Wnt/β-catenin and ERK pathways by LiCl or epidermal growth factor (EGF) treatment of osteosarcoma cells. In particular, we analyzed the mechanisms responsible for differences in cell mobility and cell proliferation when pERK or β-catenin is activated. In osteosarcoma cells treated with LiCl or EGF, active β-catenin and p-ERK protein levels were significantly increased compared to those in the control group. However, in wound healing and transwell invasion assays, U2OS and SaOS2 cell migration was significantly reduced by LiCl treatment but increased by EGF treatment. In addition, the proliferation of U2OS cells was reduced by LiCl treatment but increased by EGF treatment. Using immunofluorescence microscopy, we observed nuclear accumulation of phosphorylated ERK (pERK) with EGF treatment, but pERK was restricted to the perinuclear area with LiCl treatment. These results were confirmed using immunoblot assays after subcellular fractionation. Together, these data suggest that LiCl interferes with the translocation of pERK from the cytoplasm to the nucleus.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHBone Marrow Cells / cytology-
dc.subject.MESHBone Marrow Cells / drug effects-
dc.subject.MESHBone Marrow Cells / metabolism-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Movement / drug effects-
dc.subject.MESHCell Movement / genetics-
dc.subject.MESHCell Nucleus / drug effects-
dc.subject.MESHCell Nucleus / metabolism-
dc.subject.MESHCell Proliferation / drug effects-
dc.subject.MESHCell Proliferation / genetics-
dc.subject.MESHDiffusion Chambers, Culture-
dc.subject.MESHEpidermal Growth Factor / pharmacology*-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHGlycogen Synthase Kinase 3 beta / genetics-
dc.subject.MESHGlycogen Synthase Kinase 3 beta / metabolism-
dc.subject.MESHHumans-
dc.subject.MESHLithium Chloride / pharmacology*-
dc.subject.MESHMAP Kinase Signaling System / drug effects-
dc.subject.MESHMitogen-Activated Protein Kinase 1 / antagonists & inhibitors-
dc.subject.MESHMitogen-Activated Protein Kinase 1 / genetics*-
dc.subject.MESHMitogen-Activated Protein Kinase 1 / metabolism-
dc.subject.MESHMitogen-Activated Protein Kinase 3 / antagonists & inhibitors-
dc.subject.MESHMitogen-Activated Protein Kinase 3 / genetics*-
dc.subject.MESHMitogen-Activated Protein Kinase 3 / metabolism-
dc.subject.MESHOsteoblasts / drug effects*-
dc.subject.MESHOsteoblasts / metabolism-
dc.subject.MESHOsteoblasts / pathology-
dc.subject.MESHPhosphorylation / drug effects-
dc.subject.MESHPrimary Cell Culture-
dc.subject.MESHProtein Transport / drug effects-
dc.subject.MESHWnt Signaling Pathway / drug effects-
dc.subject.MESHbeta Catenin / genetics-
dc.subject.MESHbeta Catenin / metabolism-
dc.titleLithium chloride inhibits the migration and invasion of osteosarcoma cells by blocking nuclear translocation of phospho-Erk-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Tropica Medicine (열대의학교실)-
dc.contributor.googleauthorJu Yeong Kim-
dc.contributor.googleauthorHun Hee Park-
dc.contributor.googleauthorTai-Soon Yong-
dc.contributor.googleauthorSoung-Hoo Jeon-
dc.identifier.doi10.1016/j.bbrc.2021.10.025-
dc.contributor.localIdA00937-
dc.contributor.localIdA02424-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid34656851-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0006291X21014327-
dc.subject.keywordEpidermal growth factor-
dc.subject.keywordInvasion-
dc.subject.keywordLithium chloride-
dc.subject.keywordOsteosarcoma-
dc.subject.keywordProliferation-
dc.contributor.alternativeNameKim, Ju Young-
dc.contributor.affiliatedAuthor김주영-
dc.contributor.affiliatedAuthor용태순-
dc.citation.volume581-
dc.citation.startPage74-
dc.citation.endPage80-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.581 : 74-80, 2021-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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